FastQCFastQC Report
Thu 9 Nov 2017
HTCTCAFXX_n01_ah8643i3h-1117.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTCTCAFXX_n01_ah8643i3h-1117.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6458889
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC175040.2710063603817932TruSeq Adapter, Index 3 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG27700.035.0224611
CTTAGGC27500.034.56204233
ACTTAGG28300.033.66042332
ACACGTC29000.033.52808413
CGTCTGA29550.032.90404516
ACGTCTG29750.032.75678315
CACACGT29950.032.53829212
GATCGGA29100.031.6088711
CATCTCG27100.031.25815639
CAGTCAC31250.030.90359927
CGTATGC30900.030.90102444
AGCACAC31500.030.72793210
TTAGGCA31400.030.19970534
GAGCACA32150.030.1066829
CACGTCT32100.030.08458114
TCCAGTC32400.029.80648225
CCAGTCA32800.029.64442326
CACTTAG32850.029.26605231
AGTCACT33100.029.17658428
TAGGCAT30700.029.16829935
Produced by FastQC (version 0.11.2)