FastQCFastQC Report
Thu 9 Nov 2017
HTCTCAFXX_n01_ah8643c3htop2-1117.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTCTCAFXX_n01_ah8643c3htop2-1117.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7863122
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC794711.0106799818189265TruSeq Adapter, Index 8 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG111750.040.45171711
GATCGGA106800.040.3608321
GTCACAC112200.039.90142429
CGTCTGA113450.039.8261716
ACGTCTG113650.039.81415615
ACACGTC113950.039.74794413
CACGTCT113950.039.74794414
CACACGT113750.039.74047512
GAACTCC113750.039.43206421
AGCACAC115200.039.39303210
TCCAGTC114700.039.16375425
CAGTCAC115600.038.93496327
GAGCACA117250.038.6489
CCAGTCA116350.038.5327326
TCGGAAG118100.038.467133
GTCTGAA118050.038.3485817
AGAGCAC118650.038.2661368
AGTCACA117850.038.1918628
CGTATGC110100.038.13113444
ATCTCGT100300.037.7115740
Produced by FastQC (version 0.11.2)