FastQCFastQC Report
Thu 9 Nov 2017
HTCTCAFXX_n01_ah7797i3h-1117.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTCTCAFXX_n01_ah7797i3h-1117.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6805725
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT584290.858527195853491TruSeq Adapter, Index 16 (97% over 40bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG73380.1078209889468058No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCCG87950.041.6759235
GTCCCGA82950.041.35032336
GCACACG94100.040.70001211
ACCCGTC91350.040.4842432
CCCGTCC91900.040.2910233
GATCGGA94400.040.281641
TCACCCG91700.040.23346330
ACGTCTG95500.040.195515
CCCGATC74050.040.17197438
ACACGTC96000.039.96323413
CACCCGT92650.039.9399431
CCGTCCC92150.039.91967434
CACACGT96400.039.6833212
CGTCTGA96300.039.6331516
CACGTCT96500.039.596614
TCCCGAT79900.039.4056137
GTCACCC94250.039.2146629
AGCACAC98300.039.07294510
GAGCACA98800.038.8532269
CCGATCT76300.038.75665739
Produced by FastQC (version 0.11.2)