FastQCFastQC Report
Thu 9 Nov 2017
HTCTCAFXX_n01_ah7797i0h-1117.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTCTCAFXX_n01_ah7797i0h-1117.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6988540
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT4717606.750480071660175TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGCTCTCGTAT263150.3765450294339018TruSeq Adapter, Index 19 (97% over 44bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG200110.2863402083983207No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGAGCTCGTAT199300.285181168026512TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATATCGTAT84380.12074052663360302TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATGTCGTAT77010.11019469016418308TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA611350.043.4081841
GCACACG635300.043.25745811
TCGGAAG639550.043.05273
CACACGT638100.043.0469612
ACACGTC639150.043.0175413
ACGTCTG637750.042.99814615
GAGCACA640150.042.9784479
AGCACAC640300.042.96776210
CACGTCT638800.042.9481314
CGTCTGA637950.042.89501616
AGAGCAC642650.042.8797158
ATCGGAA643400.042.836732
AGTCACG634700.042.65237828
CTCGTAT590800.042.64077844
CGGAAGA648100.042.6035544
GAACTCC637950.042.564321
TCACGTG635000.042.52319330
GTCACGT635150.042.51631529
TCCAGTC637900.042.46854425
CACGTGA635000.042.46806731
Produced by FastQC (version 0.11.2)