FastQCFastQC Report
Thu 9 Nov 2017
HTCTCAFXX_n01_ah7797c3htop2-1117.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTCTCAFXX_n01_ah7797c3htop2-1117.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6973046
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT1215461.7430832953059539TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG153850.041.77828211
GATCGGA147700.041.7146571
CACACGT155700.041.28188312
ACACGTC156050.041.2453813
ACGTCTG155150.041.21525615
CGTCTGA154950.041.1832816
CACGTCT156150.041.04992314
AGTTCCG152200.040.82448634
TCCAGTC155300.040.73840325
AGCACAC158050.040.7101210
CAGTCAC155850.040.62286827
GAGCACA158400.040.5926869
AGAGCAC158900.040.492648
CCAGTCA156150.040.4462126
GAACTCC156950.040.40678821
CCGTATC144350.040.3970538
TCGGAAG159900.040.3133963
GTCTGAA158950.040.17457617
TATCTCG145100.040.1272941
CTCGTAT150750.040.0649944
Produced by FastQC (version 0.11.2)