FastQCFastQC Report
Thu 9 Nov 2017
HTCTCAFXX_n01_ah7797c3hhop1-1117.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTCTCAFXX_n01_ah7797c3hhop1-1117.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8497878
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT7808429.188670395126877TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCGCGTAT215250.25329852935050373TruSeq Adapter, Index 15 (97% over 40bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT126110.14840175394374924TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATATCGTAT113830.13395108755385757TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAAGCTCGTAT113490.13355098767009835TruSeq Adapter, Index 15 (97% over 40bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG107380.12636095740607242No Hit
TATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT94140.11078059722674297TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA968150.043.5355341
GCACACG1010750.043.29450211
TCGGAAG1018500.043.1948973
CACACGT1014550.043.169212
ACACGTC1017000.043.12144513
GAGCACA1018750.043.1102459
ATCGGAA1022100.043.09862
AGCACAC1016800.043.09747310
CACGTCT1015950.043.0750714
AGAGCAC1021550.043.063148
ACGTCTG1015900.043.0471315
CGTCTGA1016150.042.99107716
CGGAAGA1024450.042.935434
CTCGTAT911900.042.88215644
GAACTCC1009650.042.802121
GTCTGAA1023000.042.6881617
TCCAGTC1009950.042.55925425
GAAGAGC1034550.042.5585446
CTGAACT1022500.042.5457819
ACTCCAG1013100.042.545623
Produced by FastQC (version 0.11.2)