FastQCFastQC Report
Thu 9 Nov 2017
HTCTCAFXX_n01_ah7797c0htop2-1117.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTCTCAFXX_n01_ah7797c0htop2-1117.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6783772
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT1840442.713003915815567TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA232100.042.470991
GCACACG234850.042.43086211
ACACGTC236100.042.13199213
CGTCCGC230250.042.02489533
ACGTCCG230500.041.9851532
CACACGT237200.041.97370512
AGCACAC237800.041.93224310
AGAGCAC238950.041.868838
GAGCACA238600.041.8288359
AGTCACG233950.041.79640628
CACGTCT237600.041.79194314
CGTCTGA236550.041.7821616
ACGTCTG237700.041.7373415
TCGGAAG242300.041.5881423
GAACTCC235950.041.54376221
CACGTCC233450.041.52998731
CCGCACA221700.041.3844736
GTCACGT236000.041.3596929
GTCCGCA231100.041.35714334
TCACGTC236000.041.3236230
Produced by FastQC (version 0.11.2)