FastQCFastQC Report
Thu 9 Nov 2017
HTCTCAFXX_n01_ah7606i3h-1117.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTCTCAFXX_n01_ah7606i3h-1117.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7660065
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT929141.2129662085112856TruSeq Adapter, Index 13 (97% over 40bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG77470.10113491204056363No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG122700.041.5931411
ACACGTC125500.040.78732713
CACACGT125950.040.51987512
ACGTCTG125650.040.51131415
CACGTCT126000.040.4858114
CGTCTGA126450.040.28980616
GATCGGA122300.040.1404151
AGCACAC128000.039.99148610
GAGCACA128850.039.7788859
TCCAGTC127150.039.39507725
CACAGTC128000.039.32431831
CAGTCAC128150.039.20808427
CCAGTCA128450.038.99662826
GTCACAG129000.038.933729
CTCGTAT121800.038.92811244
AGTCACA129300.038.9106628
AGAGCAC132350.038.8931438
GAACTCC129550.038.81757721
TCGGAAG133300.038.5227283
ATCTCGT120200.038.1120542
Produced by FastQC (version 0.11.2)