FastQCFastQC Report
Thu 9 Nov 2017
HTCTCAFXX_n01_ah7606c3htop2-1117.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTCTCAFXX_n01_ah7606c3htop2-1117.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7151187
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC1677322.3455127099878665TruSeq Adapter, Index 6 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG88460.12369974383273714No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACGC196850.042.76942429
AGTCACG199100.042.5497928
TCACGCC197150.042.4594430
ACACGTC202250.042.3650813
GCACACG202200.042.34321211
GATCGGA196150.042.2945941
CACACGT203300.042.17902812
CACGCCA199000.042.09758831
ACGTCTG203250.042.08087515
CGTCTGA203100.042.0794616
CACGTCT204000.041.98008314
CGTATGC200850.041.77847344
AGCACAC206050.041.6374510
CAGTCAC203200.041.62629327
TCCAGTC204550.041.53439725
TATCTCG197250.041.4284839
GAGCACA207850.041.298039
AGAGCAC208050.041.2900548
CCAGTCA204950.041.281626
GAACTCC206500.041.22712321
Produced by FastQC (version 0.11.2)