FastQCFastQC Report
Thu 9 Nov 2017
HTCTCAFXX_n01_ah7606c3hred1-1117.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTCTCAFXX_n01_ah7606c3hred1-1117.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7191316
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC908431.2632319314017073TruSeq Adapter, Index 12 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG105120.14617630486547942No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG119450.041.74933211
ACACGTC120900.041.19374513
ACGTCTG121700.040.94103615
CACGTCT122050.040.7875814
CACACGT122500.040.7816912
CGTCTGA123100.040.43967416
GATCGGA118900.040.3446241
CGTATGC121800.040.04802344
AGCACAC125300.039.88792410
CAGTCAC124550.039.72271727
GAGCACA126450.039.6295439
TCCAGTC125000.039.57971625
GAACTCC125700.039.49848621
TCGGAAG127600.039.3492743
AGTCACC125250.039.3426428
CCAGTCA125850.039.29491426
CTCGTAT118700.038.8339142
GTCACCT126400.038.7426629
AGAGCAC129350.038.741068
TCGTATG125500.038.6930943
Produced by FastQC (version 0.11.2)