FastQCFastQC Report
Thu 9 Nov 2017
HTCTCAFXX_n01_ah7606c3hhop1-1117.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTCTCAFXX_n01_ah7606c3hhop1-1117.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7124664
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC1114091.5637088289356522TruSeq Adapter, Index 7 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG106590.14960705515375883No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG152050.041.95668411
GATCGGA148050.041.7224581
ACACGTC153700.041.634513
CACGTCT154300.041.40131414
CACACGT154700.041.2521912
CGTCTGA154750.041.1529916
ACGTCTG156150.040.84037415
CGTATGC150050.040.79139744
AGCACAC157350.040.66928510
GAACTCC155900.040.56778721
GAGCACA158100.040.559849
CATCTCG135350.040.54302639
CAGTCAC155400.040.51427
CTCGTAT142600.040.27103842
TCGGAAG159750.040.1206933
TCCAGTC157650.040.0753225
CCAGTCA156850.040.0553226
AGAGCAC160200.040.028168
TCGTATG150400.039.93775643
AGTCACC157350.039.80249428
Produced by FastQC (version 0.11.2)