FastQCFastQC Report
Thu 9 Nov 2017
HTCTCAFXX_n01_ah5187i3h-1117.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTCTCAFXX_n01_ah5187i3h-1117.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6775980
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC1443352.1300977865932307TruSeq Adapter, Index 5 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG188430.27808523637909205No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATGC91600.13518339782584954TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGAGCTCGTATGC74980.11065558044740392TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGCTCTCGTATGC67870.10016263330175118TruSeq Adapter, Index 5 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG202900.042.63967511
ACACGTC205300.042.09803813
GATCGGA197250.042.0680661
CACACGT205450.042.05690812
ACGTCTG205850.041.87869615
CGTCTGA206200.041.85059416
CGTATGC201950.041.7711544
CACGTCT206600.041.7692614
GTCACAC203550.041.73003829
GAGCACA207900.041.583069
CACACAG204850.041.5090931
AGCACAC208750.041.4450510
TCCAGTC205600.041.41881625
CAGTCAC206800.041.2103927
CCAGTCA206600.041.1225126
CACAGTG206100.041.04687533
GAACTCC208700.041.0026521
AGAGCAC211350.040.9979488
GATCTCG169350.040.99253539
TCGGAAG211800.040.8652463
Produced by FastQC (version 0.11.2)