FastQCFastQC Report
Thu 9 Nov 2017
HTCTCAFXX_n01_ah5187c3htop2-1117.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTCTCAFXX_n01_ah5187c3htop2-1117.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6959503
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC307530.4418850024204315TruSeq Adapter, Index 4 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG47350.036.794115
GCACACG47500.036.77079811
CGTCTGA47950.036.4713316
GATCGGA46500.036.340211
ACTGACC46950.036.22101632
CACGTCT48700.035.99999214
GAACTCC50100.034.86403721
ACACGTC50400.034.82935313
CACACGT51000.034.59237312
TCCAGTC50750.034.07097225
GAGCACA52250.033.7227029
CAGTCAC51250.033.6527227
CCAGTCA51700.033.48745726
AGCACAC52850.033.423110
AGTCACT51900.033.35841428
ACTCCAG52150.033.3248123
CGTATGC51150.033.12139544
AGAGCAC53550.033.068358
TCGGAAG53850.032.9299473
GTCTGAA53450.032.92444217
Produced by FastQC (version 0.11.2)