FastQCFastQC Report
Thu 9 Nov 2017
HTCTCAFXX_n01_ah5184i3h-1117.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTCTCAFXX_n01_ah5184i3h-1117.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7684460
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC2257572.9378381825138007TruSeq Adapter, Index 2 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG140810.18323994138820426No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG284450.042.69553411
ACACGTC284850.042.5660813
GATCGGA275600.042.465331
ACGTCTG285950.042.37156315
CACACGT286400.042.3667112
CACGTCT286450.042.28992514
CGTCTGA286800.042.13860316
AGCACAC289000.042.1070710
GAGCACA290500.041.912369
CGTATGC282000.041.89115544
CCGATGT283650.041.7236933
AGAGCAC292300.041.6918958
TATCTCG264300.041.65263439
GAACTCC288650.041.6034321
TCGGAAG293750.041.544733
TCCAGTC288300.041.52449425
GTCACCG287550.041.50571429
CCAGTCA288700.041.4215126
CAGTCAC289250.041.3959927
CTCGTAT276450.041.35685742
Produced by FastQC (version 0.11.2)