FastQCFastQC Report
Thu 9 Nov 2017
HTCTCAFXX_n01_ah5184c3htop2-1117.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTCTCAFXX_n01_ah5184c3htop2-1117.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6619112
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC622880.9410325735536731TruSeq Adapter, Index 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG92350.040.6842815
GCACACG93600.040.3054711
GAACTCC93400.040.0623521
CACGTCT94050.039.90211514
GATCGGA90900.039.895441
CGTCTGA94550.039.80773516
CACACGT94450.039.80312
ACACGTC94550.039.7841713
CAGTCAC95250.039.2155427
GAGCACA96100.039.2117549
CACGATC77600.038.95763436
TCCAGTC96400.038.74743325
AGCACAC97350.038.6850810
CCAGTCA96400.038.65644526
CATCACG95200.038.45630333
CGTATGC95400.038.40077644
AGAGCAC98600.038.373718
TCGGAAG97900.038.2741973
GTCACAT97550.038.24756229
ACTCCAG97350.038.21084223
Produced by FastQC (version 0.11.2)