FastQCFastQC Report
Thu 2 Jun 2022
HTCK2BGXL_n01_RA12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTCK2BGXL_n01_RA12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16993859
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGGTTTGTCCCTCTTAACCATTAAATCAGTTCTAGAACCAACAAAAAG524420.30859382792336926No Hit
CTCCGGTTCCACTCCAGCGATTTCACGTTCTCTTGAACTCTCTCTTCAAA232860.13702596920452265No Hit
CTGGAAACTTACACCTATACGCCACATTTCGAAGCAGCCAAAGACTGATC225660.1327891445962921No Hit
GCGGCTGCTGGCACCAGACTTGCCCTCTCCTTGCTACTCGAAGAATCATT220740.12989398111400124No Hit
CCGGTTTGTCCCTCTTAACCATTAAATCAGTTCTAGAACCAACAAAAAGA216330.12729892604146004No Hit
TCCGGTTCCACTCCAGCGATTTCACGTTCTCTTGAACTCTCTCTTCAAAG210750.12401538697008137No Hit
CTCGGATGGAGCGTCTTGTCCTGGCTGTCCTGGGGCTCCTGGGGCTCCTG210700.12398596457696864No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)