FastQCFastQC Report
Thu 2 Jun 2022
HTCK2BGXL_n01_RA10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTCK2BGXL_n01_RA10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14049623
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACATTGAACCAGAGTGATCTGGCGTTGGCAAGCGTCGCAGAATTCACT612200.4357412294977595No Hit
CCGACATTGAACCAGAGTGATCTGGCGTTGGCAAGCGTCGCAGAATTCAC588170.41863756771267097No Hit
GCGATTTCACGTTCTCTTGAACTCTCTCTTCAAAGTCCTTTGCAACTTTC438550.3121436069850415No Hit
CTTGACACTGTCTTCGAGGAGAGTCACGGTGCCATAAAATGGCTCCGCTT435990.3103214940358186No Hit
CTCAAATGGTAGCTTCAGACCATTGATCTGTCGATCAAGTCTATGAACCA403810.28741696485379004No Hit
GTCAAACTCCCCGCTTGACACTGTCTTCGAGGAGAGTCACGGTGCCATAA386110.2748187620408035No Hit
GCCGTTTCTAAGGCTCCCTCTCCGGAGTCGAACCCTTATTCTCCGTTACC346890.24690342224841194No Hit
CGAAGATTCGCCAACTATTTAGCAGGCTAGAGTCTCGCTCGTTATCGGAA328230.23362192707946683No Hit
CGAACCCGAAGATTCGCCAACTATTTAGCAGGCTAGAGTCTCGCTCGTTA195450.13911405309594427No Hit
CCGAAGTCCTGTTTCATTATTCCATGATGAGCTATTCAAGCAAGCGCTTG182780.13009601752303246No Hit
CGGACATCTAAGGGCATCACAGACCTGTTATCGCTCAATCTCGTGCGGCT164300.11694263967082962No Hit
CCGGTTTGTCCCTCTTAACCATTAAATCAGTTCTAGAACCAACAAAAAGA146440.10423055479851667No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)