FastQCFastQC Report
Thu 2 Jun 2022
HTCK2BGXL_n01_RA07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTCK2BGXL_n01_RA07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13952336
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCAAACTCCCCGCTTGACACTGTCTTCGAGGAGAGTCACGGTGCCATAA630290.4517451414587492No Hit
CCGGTTTGTCCCTCTTAACCATTAAATCAGTTCTAGAACCAACAAAAAGA476450.3414840353615337No Hit
CCGACATTGAACCAGAGTGATCTGGCGTTGGCAAGCGTCGCAGAATTCAC424430.30419995619371554No Hit
GTGATTTAGCGCCCCGACATTGAACCAGAGTGATCTGGCGTTGGCAAGCG351390.25185029947673276No Hit
TTCCACTCCAGCGATTTCACGTTCTCTTGAACTCTCTCTTCAAAGTCCTT304710.21839353639419237No Hit
CTCGTGTTGCCACGAGATAGAGATGCCTCCCGACACCGGGAAGCAAGAAA230780.16540599366299666No Hit
ACGAACCCGAAGATTCGCCAACTATTTAGCAGGCTAGAGTCTCGCTCGTT227840.1632988196385179No Hit
GCGATTTCACGTTCTCTTGAACTCTCTCTTCAAAGTCCTTTGCAACTTTC225310.16148550321609226No Hit
CGAACCCGAAGATTCGCCAACTATTTAGCAGGCTAGAGTCTCGCTCGTTA219650.1574288348560413No Hit
GCTCAATCTCGTGCGGCTAAACACCGCTTATCCCTCTAAGAAGTTATACG214840.15398138347585666No Hit
CTGAGATCCAGCTATCCTGAGGGAAACTTCGGAAGGAACCAGCTACTAGA201360.14431991890103565No Hit
CTCAATCTCGTGCGGCTAAACACCGCTTATCCCTCTAAGAAGTTATACGA191270.1370881549870932No Hit
CAAACTCCCCGCTTGACACTGTCTTCGAGGAGAGTCACGGTGCCATAAAA186430.13361920183114856No Hit
CCGGAGTCGAACCCTTATTCTCCGTTACCCGTAACAACCATGGTAGTCCA177300.1270754947415257No Hit
CGACATTGAACCAGAGTGATCTGGCGTTGGCAAGCGTCGCAGAATTCACT172800.12385022837752761No Hit
AGCGATTTCACGTTCTCTTGAACTCTCTCTTCAAAGTCCTTTGCAACTTT172230.12344169463808785No Hit
CGCGCTTGTGCTCCGAAGAACACAATCTTTGCATCTCAGTTAGGGAATAT166140.1190768341588104No Hit
CTTGGCTGTGGTTTCGCTAGAAAGTAGTTAGGGACAGTAGGAATCTCGTT161720.11590890586350559No Hit
GTTTGTCCCTCTTAACCATTAAATCAGTTCTAGAACCAACAAAAAGAACC148720.10659146970084436No Hit
CTCAAATGGTAGCTTCAGACCATTGATCTGTCGATCAAGTCTATGAACCA146650.10510784717340522No Hit
GCCGTTTCTAAGGCTCCCTCTCCGGAGTCGAACCCTTATTCTCCGTTACC142510.10214060211852696No Hit
GTTCAGTTATAATCTCTCAAATGGTAGCTTCAGACCATTGATCTGTCGAT141830.1016532285346339No Hit
CGGACATCTAAGGGCATCACAGACCTGTTATCGCTCAATCTCGTGCGGCT141760.10160305772452728No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)