FastQCFastQC Report
Thu 2 Jun 2022
HTCK2BGXL_n01_RA06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTCK2BGXL_n01_RA06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14911372
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCAAACTCCCCGCTTGACACTGTCTTCGAGGAGAGTCACGGTGCCATAA781100.5238283908415671No Hit
CCGGTTTGTCCCTCTTAACCATTAAATCAGTTCTAGAACCAACAAAAAGA612100.410492072761648No Hit
CCGACATTGAACCAGAGTGATCTGGCGTTGGCAAGCGTCGCAGAATTCAC477280.3200778573561172No Hit
CTCGTGTTGCCACGAGATAGAGATGCCTCCCGACACCGGGAAGCAAGAAA358850.2406552529170354No Hit
TTCCACTCCAGCGATTTCACGTTCTCTTGAACTCTCTCTTCAAAGTCCTT345230.23152128456053542No Hit
GTGATTTAGCGCCCCGACATTGAACCAGAGTGATCTGGCGTTGGCAAGCG337330.226223314662125No Hit
GCGATTTCACGTTCTCTTGAACTCTCTCTTCAAAGTCCTTTGCAACTTTC283400.19005628724171056No Hit
GTTTGTCCCTCTTAACCATTAAATCAGTTCTAGAACCAACAAAAAGAACC236410.15854342578268452No Hit
AGCGATTTCACGTTCTCTTGAACTCTCTCTTCAAAGTCCTTTGCAACTTT228200.15303756086294407No Hit
GCTCAATCTCGTGCGGCTAAACACCGCTTATCCCTCTAAGAAGTTATACG219120.1469482486252774No Hit
CTGAGATCCAGCTATCCTGAGGGAAACTTCGGAAGGAACCAGCTACTAGA214650.1439505365435186No Hit
CGAACCCGAAGATTCGCCAACTATTTAGCAGGCTAGAGTCTCGCTCGTTA204050.13684186807223372No Hit
CTCAATCTCGTGCGGCTAAACACCGCTTATCCCTCTAAGAAGTTATACGA202870.1360505257329775No Hit
CAAACTCCCCGCTTGACACTGTCTTCGAGGAGAGTCACGGTGCCATAAAA195530.13112810813116324No Hit
ACGAACCCGAAGATTCGCCAACTATTTAGCAGGCTAGAGTCTCGCTCGTT188060.1261185087462106No Hit
CGGACATCTAAGGGCATCACAGACCTGTTATCGCTCAATCTCGTGCGGCT186920.12535399157099697No Hit
CCGGAGTCGAACCCTTATTCTCCGTTACCCGTAACAACCATGGTAGTCCA179840.1206059375354595No Hit
GCCGTTTCTAAGGCTCCCTCTCCGGAGTCGAACCCTTATTCTCCGTTACC173260.11619319805045439No Hit
CGACATTGAACCAGAGTGATCTGGCGTTGGCAAGCGTCGCAGAATTCACT169070.11338326211699366No Hit
CTCTCGTGTTGCCACGAGATAGAGATGCCTCCCGACACCGGGAAGCAAGA154910.10388715404591878No Hit
GTTCAGTTATAATCTCTCAAATGGTAGCTTCAGACCATTGATCTGTCGAT154590.1036725527335781No Hit
CTTGGCTGTGGTTTCGCTAGAAAGTAGTTAGGGACAGTAGGAATCTCGTT154210.10341771367517354No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)