FastQCFastQC Report
Thu 2 Jun 2022
HTCK2BGXL_n01_RA05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTCK2BGXL_n01_RA05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15562297
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCAAACTCCCCGCTTGACACTGTCTTCGAGGAGAGTCACGGTGCCATAA1036950.6663219446332376No Hit
CCGACATTGAACCAGAGTGATCTGGCGTTGGCAAGCGTCGCAGAATTCAC772710.4965269587131No Hit
CCGGTTTGTCCCTCTTAACCATTAAATCAGTTCTAGAACCAACAAAAAGA749870.4818504620494006No Hit
GCGATTTCACGTTCTCTTGAACTCTCTCTTCAAAGTCCTTTGCAACTTTC496590.31909813827611694No Hit
TTCCACTCCAGCGATTTCACGTTCTCTTGAACTCTCTCTTCAAAGTCCTT478760.3076409607142185No Hit
CGACATTGAACCAGAGTGATCTGGCGTTGGCAAGCGTCGCAGAATTCACT420770.2703778240448695No Hit
CGAACCCGAAGATTCGCCAACTATTTAGCAGGCTAGAGTCTCGCTCGTTA392470.2521928478810037No Hit
CTCAAATGGTAGCTTCAGACCATTGATCTGTCGATCAAGTCTATGAACCA384340.24696868335053623No Hit
GTGATTTAGCGCCCCGACATTGAACCAGAGTGATCTGGCGTTGGCAAGCG384000.24675020660510466No Hit
AGCGATTTCACGTTCTCTTGAACTCTCTCTTCAAAGTCCTTTGCAACTTT379910.24412205987329505No Hit
CTGAGATCCAGCTATCCTGAGGGAAACTTCGGAAGGAACCAGCTACTAGA329640.21181963048256952No Hit
CAAACTCCCCGCTTGACACTGTCTTCGAGGAGAGTCACGGTGCCATAAAA328640.21117705181953536No Hit
GTTTGTCCCTCTTAACCATTAAATCAGTTCTAGAACCAACAAAAAGAACC313850.20167331339326064No Hit
CTCAATCTCGTGCGGCTAAACACCGCTTATCCCTCTAAGAAGTTATACGA281460.18086019049758528No Hit
CTCGTGTTGCCACGAGATAGAGATGCCTCCCGACACCGGGAAGCAAGAAA274910.17665130025471176No Hit
CGAAGATTCGCCAACTATTTAGCAGGCTAGAGTCTCGCTCGTTATCGGAA250140.16073462677135644No Hit
CGCGCTTGTGCTCCGAAGAACACAATCTTTGCATCTCAGTTAGGGAATAT249890.1605739821055979No Hit
GCCGTTTCTAAGGCTCCCTCTCCGGAGTCGAACCCTTATTCTCCGTTACC242960.1561209119707714No Hit
ACGAACCCGAAGATTCGCCAACTATTTAGCAGGCTAGAGTCTCGCTCGTT241900.15543977858795524No Hit
GCTCAATCTCGTGCGGCTAAACACCGCTTATCCCTCTAAGAAGTTATACG241650.15527913392219672No Hit
GCAAGCTCCACGCCTTGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAG237600.1526766903369085No Hit
CTTGGCTGTGGTTTCGCTAGAAAGTAGTTAGGGACAGTAGGAATCTCGTT234800.15087747008041294No Hit
CTTGACACTGTCTTCGAGGAGAGTCACGGTGCCATAAAATGGCTCCGCTT231230.1485834642533811No Hit
CGGACATCTAAGGGCATCACAGACCTGTTATCGCTCAATCTCGTGCGGCT221960.14262676004705477No Hit
CAGACTTGCCCTCTCCTTGCTACTCGAAGAATCATTTGTATTCAACTCAT219460.14102031338946944No Hit
CCGGAGTCGAACCCTTATTCTCCGTTACCCGTAACAACCATGGTAGTCCA191240.12288674351864637No Hit
ACCAGACTTGCCCTCTCCTTGCTACTCGAAGAATCATTTGTATTCAACTC178720.11484165865745911No Hit
CTTCAAAGTCCTTTGCAACTTTCCCTCACGGTACTTGTTCGCTATCGCAA178370.11461675612539717No Hit
GTCAATATCAACCAACACCTTTTATGGGGTCTCATGAGCGAGAAGTTAGG173660.11159021062250643No Hit
GCAAATTAATAGCCTCCCTTGGTGTTTTAAGGGCCGTTCAAAGAGCACGA172820.11105044454555776No Hit
CAAATTAATAGCCTCCCTTGGTGTTTTAAGGGCCGTTCAAAGAGCACGAG156470.1005442834049498No Hit
AAACTCCCCGCTTGACACTGTCTTCGAGGAGAGTCACGGTGCCATAAAAT156330.10045432239212501No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)