FastQCFastQC Report
Thu 2 Jun 2022
HTCK2BGXL_n01_RA03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTCK2BGXL_n01_RA03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14793180
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACATTGAACCAGAGTGATCTGGCGTTGGCAAGCGTCGCAGAATTCAC695870.4703991974680224No Hit
GTCAAACTCCCCGCTTGACACTGTCTTCGAGGAGAGTCACGGTGCCATAA642240.43414600511857493No Hit
CCGGTTTGTCCCTCTTAACCATTAAATCAGTTCTAGAACCAACAAAAAGA354210.23944141827517818No Hit
CGACATTGAACCAGAGTGATCTGGCGTTGGCAAGCGTCGCAGAATTCACT303120.20490523335753366No Hit
GCGATTTCACGTTCTCTTGAACTCTCTCTTCAAAGTCCTTTGCAACTTTC298920.2020660872104578No Hit
TTCCACTCCAGCGATTTCACGTTCTCTTGAACTCTCTCTTCAAAGTCCTT256880.17364758625258395No Hit
CTCAAATGGTAGCTTCAGACCATTGATCTGTCGATCAAGTCTATGAACCA255890.1729783589464875No Hit
CGAACCCGAAGATTCGCCAACTATTTAGCAGGCTAGAGTCTCGCTCGTTA242290.16378493332738464No Hit
GTGATTTAGCGCCCCGACATTGAACCAGAGTGATCTGGCGTTGGCAAGCG222930.15069782156372058No Hit
AGCGATTTCACGTTCTCTTGAACTCTCTCTTCAAAGTCCTTTGCAACTTT210340.14218714299427168No Hit
CTCGTGTTGCCACGAGATAGAGATGCCTCCCGACACCGGGAAGCAAGAAA201540.13623845582896985No Hit
CAAACTCCCCGCTTGACACTGTCTTCGAGGAGAGTCACGGTGCCATAAAA191670.1295664623833415No Hit
CTGAGATCCAGCTATCCTGAGGGAAACTTCGGAAGGAACCAGCTACTAGA190780.12896483379503257No Hit
CGCGCTTGTGCTCCGAAGAACACAATCTTTGCATCTCAGTTAGGGAATAT171060.11563436664733344No Hit
CTTGACACTGTCTTCGAGGAGAGTCACGGTGCCATAAAATGGCTCCGCTT166230.11236934857819618No Hit
CTCAATCTCGTGCGGCTAAACACCGCTTATCCCTCTAAGAAGTTATACGA164410.1111390519144633No Hit
GCCGTTTCTAAGGCTCCCTCTCCGGAGTCGAACCCTTATTCTCCGTTACC156530.10581227295280662No Hit
GTTTGTCCCTCTTAACCATTAAATCAGTTCTAGAACCAACAAAAAGAACC153150.10352743629158843No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)