FastQCFastQC Report
Thu 2 Jun 2022
HTCK2BGXL_n01_RA02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTCK2BGXL_n01_RA02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13818253
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACATTGAACCAGAGTGATCTGGCGTTGGCAAGCGTCGCAGAATTCAC757300.5480432294878376No Hit
GTCAAACTCCCCGCTTGACACTGTCTTCGAGGAGAGTCACGGTGCCATAA704330.5097098743234764No Hit
CCGGTTTGTCCCTCTTAACCATTAAATCAGTTCTAGAACCAACAAAAAGA441720.3196641427827382No Hit
GCGATTTCACGTTCTCTTGAACTCTCTCTTCAAAGTCCTTTGCAACTTTC403180.29177349698257804No Hit
CGACATTGAACCAGAGTGATCTGGCGTTGGCAAGCGTCGCAGAATTCACT355220.25706578103614114No Hit
CTCAAATGGTAGCTTCAGACCATTGATCTGTCGATCAAGTCTATGAACCA311850.22567975850492822No Hit
TTCCACTCCAGCGATTTCACGTTCTCTTGAACTCTCTCTTCAAAGTCCTT296520.21458573670636946No Hit
CGAACCCGAAGATTCGCCAACTATTTAGCAGGCTAGAGTCTCGCTCGTTA288660.20889760811297928No Hit
AGCGATTTCACGTTCTCTTGAACTCTCTCTTCAAAGTCCTTTGCAACTTT268410.19424307833993198No Hit
CTGAGATCCAGCTATCCTGAGGGAAACTTCGGAAGGAACCAGCTACTAGA258850.18732469292608842No Hit
GTGATTTAGCGCCCCGACATTGAACCAGAGTGATCTGGCGTTGGCAAGCG255190.18467602235970063No Hit
GCCGTTTCTAAGGCTCCCTCTCCGGAGTCGAACCCTTATTCTCCGTTACC215910.15624985300240196No Hit
CAAACTCCCCGCTTGACACTGTCTTCGAGGAGAGTCACGGTGCCATAAAA213360.15440446777172195No Hit
CTCAATCTCGTGCGGCTAAACACCGCTTATCCCTCTAAGAAGTTATACGA210280.1521755318852535No Hit
CTCGTGTTGCCACGAGATAGAGATGCCTCCCGACACCGGGAAGCAAGAAA195070.14116835174460912No Hit
CGCGCTTGTGCTCCGAAGAACACAATCTTTGCATCTCAGTTAGGGAATAT195060.1411611149397829No Hit
CTTGACACTGTCTTCGAGGAGAGTCACGGTGCCATAAAATGGCTCCGCTT194610.14083545872260406No Hit
GTTTGTCCCTCTTAACCATTAAATCAGTTCTAGAACCAACAAAAAGAACC192070.13899731029675025No Hit
CGGACATCTAAGGGCATCACAGACCTGTTATCGCTCAATCTCGTGCGGCT180190.13039998616322918No Hit
CGAAGATTCGCCAACTATTTAGCAGGCTAGAGTCTCGCTCGTTATCGGAA175440.12696250387078598No Hit
CAGACTTGCCCTCTCCTTGCTACTCGAAGAATCATTTGTATTCAACTCAT172990.1251894866883679No Hit
CCGGAGTCGAACCCTTATTCTCCGTTACCCGTAACAACCATGGTAGTCCA165200.11955201572876108No Hit
GCTCAATCTCGTGCGGCTAAACACCGCTTATCCCTCTAAGAAGTTATACG156630.11335007399271095No Hit
CTTGGCTGTGGTTTCGCTAGAAAGTAGTTAGGGACAGTAGGAATCTCGTT153340.1109691652048924No Hit
ACGAACCCGAAGATTCGCCAACTATTTAGCAGGCTAGAGTCTCGCTCGTT152480.11034679998983954No Hit
GCAAGCTCCACGCCTTGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAG146460.10599024348446942No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)