FastQCFastQC Report
Thu 2 Jun 2022
HTCK2BGXL_n01_RA01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTCK2BGXL_n01_RA01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13288582
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCAAACTCCCCGCTTGACACTGTCTTCGAGGAGAGTCACGGTGCCATAA568570.4278635598591332No Hit
CCGGTTTGTCCCTCTTAACCATTAAATCAGTTCTAGAACCAACAAAAAGA539720.4061531922668649No Hit
GTGATTTAGCGCCCCGACATTGAACCAGAGTGATCTGGCGTTGGCAAGCG534620.4023153109940549No Hit
GTTCAGTTATAATCTCTCAAATGGTAGCTTCAGACCATTGATCTGTCGAT240310.18083946052332747No Hit
GCTCAATCTCGTGCGGCTAAACACCGCTTATCCCTCTAAGAAGTTATACG238130.17919895441063616No Hit
CTCGTGTTGCCACGAGATAGAGATGCCTCCCGACACCGGGAAGCAAGAAA219030.16482571278109281No Hit
CTCTCGTGTTGCCACGAGATAGAGATGCCTCCCGACACCGGGAAGCAAGA211210.1589409614961175No Hit
TTCCACTCCAGCGATTTCACGTTCTCTTGAACTCTCTCTTCAAAGTCCTT197850.14888721761283485No Hit
CCGGAGTCGAACCCTTATTCTCCGTTACCCGTAACAACCATGGTAGTCCA195090.14681024657107886No Hit
CCGACATTGAACCAGAGTGATCTGGCGTTGGCAAGCGTCGCAGAATTCAC195050.14678014554148816No Hit
CTGAGATCCAGCTATCCTGAGGGAAACTTCGGAAGGAACCAGCTACTAGA191170.14386034567119352No Hit
ACGAACCCGAAGATTCGCCAACTATTTAGCAGGCTAGAGTCTCGCTCGTT182800.13756170522934652No Hit
GCTGGCACCAGACTTGCCCTCTCCTTGCTACTCGAAGAATCATTTGTATT173460.13053311481992586No Hit
CAAACTCCCCGCTTGACACTGTCTTCGAGGAGAGTCACGGTGCCATAAAA152080.11444411450371454No Hit
CTTTGCAACCATACTACCCCCGGAACCGAAAGACTTTCGTTTCCGGATAG135190.10173395475905556No Hit
CTTGGCTGTGGTTTCGCTAGAAAGTAGTTAGGGACAGTAGGAATCTCGTT135140.10169632847206722No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)