FastQCFastQC Report
Thu 14 Jul 2022
HTC5HAFX3_n02_as5-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC5HAFX3_n02_as5-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8432465
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTAAATAAGGACATGACACATGCGAATCATACGTTTATTATTGATAAG169330.20080723726692015No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC141500.167803839090942Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG138190.1638785337383553No Hit
TCGTGTAGGGAAAGAGTGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT120860.14332701054792402Illumina Single End PCR Primer 1 (100% over 31bp)
TCGTGTAGGGAAAGAGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG119620.1418565034067737Illumina Single End PCR Primer 1 (100% over 32bp)
TGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG84510.1002198052408163Illumina Single End PCR Primer 1 (100% over 48bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)