FastQCFastQC Report
Thu 14 Jul 2022
HTC5HAFX3_n02_as5-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC5HAFX3_n02_as5-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7120767
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC131140.18416555407584603Illumina Single End PCR Primer 1 (100% over 50bp)
CGCTAAATAAGGACATGACACATGCGAATCATACGTTTATTATTGATAAG112590.15811498957907202No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG86150.12098415802679684No Hit
CAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC82230.11547913307653516Illumina Single End PCR Primer 1 (100% over 49bp)
TCGTGTAGGGAAAGAGTGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT75590.10615429489547966Illumina Single End PCR Primer 1 (100% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)