FastQCFastQC Report
Thu 14 Jul 2022
HTC5HAFX3_n02_as5-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC5HAFX3_n02_as5-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7617697
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC295020.38728240306748873Illumina Single End PCR Primer 1 (100% over 50bp)
CAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC116330.15271019574551206Illumina Single End PCR Primer 1 (100% over 49bp)
CGCTAAATAAGGACATGACACATGCGAATCATACGTTTATTATTGATAAG105570.13858519182372311No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG91110.11960307688793609No Hit
TCGTGTAGGGAAAGAGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG86040.11294752206605224Illumina Single End PCR Primer 1 (100% over 32bp)
TCGTGTAGGGAAAGAGTGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT83230.10925874316082668Illumina Single End PCR Primer 1 (100% over 31bp)
TGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG78980.10367962915826134Illumina Single End PCR Primer 1 (100% over 48bp)
CGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG77780.10210434991047819Illumina Single End PCR Primer 1 (100% over 48bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)