FastQCFastQC Report
Thu 14 Jul 2022
HTC5HAFX3_n02_as5-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC5HAFX3_n02_as5-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5039810
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC154370.3063012296098464Illumina Single End PCR Primer 1 (100% over 50bp)
CGCTAAATAAGGACATGACACATGCGAATCATACGTTTATTATTGATAAG91440.18143541125558305No Hit
TCGTGTAGGGAAAGAGTGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT82480.16365696325853554Illumina Single End PCR Primer 1 (100% over 31bp)
TCGTGTAGGGAAAGAGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG78720.1561963645454888Illumina Single End PCR Primer 1 (100% over 32bp)
CGTGTAGGGAAAGAGTGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG53330.1058174812145696Illumina Single End PCR Primer 1 (100% over 32bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)