FastQCFastQC Report
Thu 14 Jul 2022
HTC5HAFX3_n01_as5-8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC5HAFX3_n01_as5-8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9576548
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTA440300.45976901071241955TruSeq Adapter, Index 22 (97% over 40bp)
GAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGT180690.1886796787318353TruSeq Adapter, Index 22 (97% over 40bp)
ACACTCTTTCCCTACACGAAGATCGGAAGAGCACACGTCTGAACTCCAGT142900.14921869550489383Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CACTCTTTCCCTACACGAAGATCGGAAGAGCACACGTCTGAACTCCAGTC128280.13395223414533086Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCG98320.10266747475186258TruSeq Adapter, Index 22 (97% over 40bp)
CGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCG97870.1021975768303986TruSeq Adapter, Index 22 (97% over 40bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)