FastQCFastQC Report
Thu 14 Jul 2022
HTC5HAFX3_n01_as5-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC5HAFX3_n01_as5-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7120767
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTA127520.17908183205545133TruSeq Adapter, Index 18 (97% over 40bp)
GAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGT81450.11438374545888104TruSeq Adapter, Index 18 (97% over 40bp)
ACACTCTTTCCCTACACGAAGATCGGAAGAGCACACGTCTGAACTCCAGT76620.10760076828802291Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)