FastQCFastQC Report
Thu 14 Jul 2022
HTC5HAFX3_n01_as5-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC5HAFX3_n01_as5-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13908409
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG488140.35096753338214315TruSeq Adapter, Index 12 (100% over 49bp)
GAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTAT179760.12924555209729596TruSeq Adapter, Index 12 (100% over 48bp)
CAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTA177540.12764939541251627TruSeq Adapter, Index 12 (100% over 47bp)
CGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTA166070.11940258587448786TruSeq Adapter, Index 12 (100% over 47bp)
ACACTCTTTCCCTACACGAAGATCGGAAGAGCACACGTCTGAACTCCAGT159480.11466444508498419Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CACTCTTTCCCTACACGAAGATCGGAAGAGCACACGTCTGAACTCCAGTC153630.11045835652374043Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTC140450.10098207494473309TruSeq Adapter, Index 12 (100% over 44bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)