FastQCFastQC Report
Thu 14 Jul 2022
HTC5HAFX3_n01_as5-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC5HAFX3_n01_as5-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7617697
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG306570.4024444658274016TruSeq Adapter, Index 11 (100% over 49bp)
GAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTAT128570.16877804407290023TruSeq Adapter, Index 11 (100% over 48bp)
CACTCTTTCCCTACACGAAGATCGGAAGAGCACACGTCTGAACTCCAGTC90900.11932740301957402Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACACTCTTTCCCTACACGAAGATCGGAAGAGCACACGTCTGAACTCCAGT89200.11709575741854789Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTA84200.11053209388611808TruSeq Adapter, Index 11 (100% over 47bp)
CGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTA82650.10849735819106483TruSeq Adapter, Index 11 (100% over 47bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)