FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate8_94.352000000610f4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate8_94.352000000610f4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1020
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA242.3529411764705883No Hit
TCCTTGCAGTAACGTACTCTGCGTTGATACCAC50.49019607843137253No Hit
CTCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.39215686274509803No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT30.29411764705882354No Hit
CCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.29411764705882354No Hit
GAGTACGTTACTGCAAAAAAAAAAAAAAAAAAA30.29411764705882354No Hit
GTGAATGGGGTACGTTACTGCAAAAAAAAAAAA30.29411764705882354No Hit
GTACGTTACTGCAAAAAAAAAAAAAAAAAAAAA30.29411764705882354No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA30.29411764705882354No Hit
CTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.19607843137254902No Hit
GGCCACTCCTAAAAAAAAAAAAAAAAAAAAAAA20.19607843137254902No Hit
ACACATAGCCTCCTTGCAGTAACGTACTCTGCG20.19607843137254902No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA20.19607843137254902No Hit
GGGCACCTCCAAAAAAAAAAAAAAAAAAAAAAA20.19607843137254902No Hit
GTGAATGGGGACGCTCCAAAAAAAAAAAAAAAA20.19607843137254902No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA20.19607843137254902No Hit
AAAAAAATGAAAAAAAAAAAAAAAAAAAAAAAA20.19607843137254902No Hit
GGGCTCTCCAAAAAAAAAAAAAAAAAAAAAAAA20.19607843137254902No Hit
CCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.19607843137254902No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA20.19607843137254902No Hit
ACGCAGAGTGAATGGGGTACGTTACTGCAAAAA20.19607843137254902No Hit
CCTCCTTGCAGTAACGTACTCTGCGTTGATACC20.19607843137254902No Hit
CAACGCAGAGTGAATGGGGGGCCTCCAAAAAAA20.19607843137254902No Hit
GTGAATGGGTACGTTACTGCAAAAAAAAAAAAA20.19607843137254902No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACC20.19607843137254902No Hit
CTCCTTGCAGTAACGTACTCTGCGTTGATACCA20.19607843137254902No Hit
TGCCCCTCCTCCTTGCAGTAACGTACTCTGCGT20.19607843137254902No Hit
CCAAGAGCCTCCTTGCAGTAACGTACTCTGCGT20.19607843137254902No Hit
ACGCAGAGTGAATGGGAGCCCTCCAAAAAAAAA20.19607843137254902No Hit
ATCAACGCAGAGTGAATGGGGTGGCTCCAAAAA20.19607843137254902No Hit
GTATCAACGCAGAGTGAATGGGGGTCCTCCAAA20.19607843137254902No Hit
GTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.19607843137254902No Hit
AGCCCGCGCCTCCTTGCAGTAACGTACTCTGCG20.19607843137254902No Hit
ATATACCGACTCCTTGCAGTAACGTACTCTGCG20.19607843137254902No Hit
TATCAACGCAGAGTGAATGGGGCACCTCCAAAA20.19607843137254902No Hit
AACGCAGAGTGAATGGGGAGTACGTTACTGCAA20.19607843137254902No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTCCTT253.5798657E-426.9999988
TCTGCGT451.02748E-521.027
CTCTGCG401.6793283E-420.2526
CTCCTTG554.9155416E-517.1818189
GTACTCT507.6734764E-416.19999923
ACTCTGC507.6734764E-416.19999925
CGTACTC507.6734764E-416.19999922
TACTCTG507.6734764E-416.19999924
TCCTTGC609.664066E-515.7510
TTGCAGT609.664066E-515.7513
TAACGTA609.664066E-515.7519
CAGTAAC609.664066E-515.7516
CCTTGCA609.664066E-515.7511
GCAGTAA609.664066E-515.7515
AGTAACG609.664066E-515.7517
CTTGCAG609.664066E-515.7512
TGCAGTA609.664066E-515.7514
GTAACGT609.664066E-515.7518
AACGTAC550.001463746714.72727320
ACGTACT550.001463746714.72727321
Produced by FastQC (version 0.11.2)