FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate8_8.3520000006062a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate8_8.3520000006062a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23223
Sequences flagged as poor quality0
Sequence length33
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGAGCACTGTACGTACTCTGCGTTGATACCA6272.699909572406666No Hit
GCACTGTACGTACAAAAAAAAAAAAAAAAAAAA2331.0033156784222537No Hit
TATCAACGCAGAGTGAATGGGCACTGTACGTAC1900.8181544158808078No Hit
GCACTGTACGTACTCAAAAAAAAAAAAAAAAAA1540.66313568445076No Hit
CCCATGAGCACTGTACGTACTCTGCGTTGATAC1340.5770141669896224No Hit
GTGAATGGGCACTGTACGTACAAAAAAAAAAAA1210.5210351806398829No Hit
GTACGTACTCAAAAAAAAAAAAAAAAAAAAAAA1060.4564440425440296No Hit
GTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1060.4564440425440296No Hit
CTGTACGTACAAAAAAAAAAAAAAAAAAAAAAA1040.4478318907979158No Hit
CTGTACGTACTCAAAAAAAAAAAAAAAAAAAAA970.4176893596865177No Hit
GTATCAACGCAGAGTGAATGGGCACTGTACGTA920.39615898032123326No Hit
GTACGTACAAAAAAAAAAAAAAAAAAAAAAAAA900.38754682857511946No Hit
ACGCAGAGTGAATGGGCACTGTACGTACAAAAA870.37462860095594885No Hit
GTACTACTCAAAAAAAAAAAAAAAAAAAAAAAA810.3487921457176075No Hit
GTATCATCGCAGAGTGAATGGGCACTGTACGTA720.3100374628600956No Hit
CCATGAGCACTGTACGTACTCTGCGTTGATACC710.3057313869870387No Hit
GAGTGAATGGGCACTGTACGTACAAAAAAAAAA620.26697670412952673No Hit
GTACTACAAAAAAAAAAAAAAAAAAAAAAAAAA580.2497524006372992No Hit
GTACGTACCTAAAAAAAAAAAAAAAAAAAAAAA570.24544632476424236No Hit
AACGCAGAGTGAATGGGCACTGTACGTACAAAA540.2325280971450717No Hit
GAATGGGCACTGTACGTACAAAAAAAAAAAAAA490.21099771777978726No Hit
GCCATGAGCACTGTACGTACTCTGCGTTGATAC400.17224303492227533No Hit
GCACTGTACGTACCTAAAAAAAAAAAAAAAAAA390.16793695904921843No Hit
ACCATGAGCACTGTACGTACTCTGCGTTGATAC390.16793695904921843No Hit
GTGAATGGGCACTGTACGTAAAAAAAAAAAAAA380.16363088317616156No Hit
GCACTGTACGTAAAAAAAAAAAAAAAAAAAAAA370.15932480730310467No Hit
CTCATGAGCACTGTACGTACTCTGCGTTGATAC370.15932480730310467No Hit
TATCTACGCAGAGTGAATGGGCACTGTACGTAC360.1550187314300478No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA350.15071265555699093No Hit
GTGAATGGGCACTGTACGTACTCAAAAAAAAAA340.14640657968393403No Hit
GCACTGTACGTACCAAAAAAAAAAAAAAAAAAA340.14640657968393403No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA300.1291822761917065No Hit
GGTATCAACGCAGAGTGAATGGGCACTGTACGT300.1291822761917065No Hit
ACGCAGAGTGAATGGGCACTGTACGTAAAAAAA290.1248762003186496No Hit
CACTGTACGTACTCAAAAAAAAAAAAAAAAAAA290.1248762003186496No Hit
GCACTGTACGTACTAAAAAAAAAAAAAAAAAAA290.1248762003186496No Hit
TATCAACGCAGAGTGAATGGGCACTGTACGTAA290.1248762003186496No Hit
GTGAATGGGCACTGTACAAAAAAAAAAAAAAAA280.12057012444559273No Hit
CAACTCAGAGTGAATGGGCACTGTACGTACAAA280.12057012444559273No Hit
GCATGAGCACTGTACGTACTCTGCGTTGATACC280.12057012444559273No Hit
CCCCCCATGAGCACTGTACGTACTCTGCGTTGA270.11626404857253585No Hit
AATGGGCACTGTACGTACAAAAAAAAAAAAAAA270.11626404857253585No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT270.11626404857253585No Hit
GAGTGAATGGGCACTGTACGTACTCAAAAAAAA270.11626404857253585No Hit
GTACGTAAAAAAAAAAAAAAAAAAAAAAAAAAA260.11195797269947895No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA260.11195797269947895No Hit
CACTGTACGTACAAAAAAAAAAAAAAAAAAAAA260.11195797269947895No Hit
CCCCCCCATGAGCACTGTACGTACTCTGCGTTG260.11195797269947895No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA260.11195797269947895No Hit
GAGCACTGTACGTACTCTGCGTTGATACCACTG250.10765189682642208No Hit
ATCAACGCAGAGTGAATGGGCACTGTACGTACA250.10765189682642208No Hit
GTACGTACCTGAAAAAAAAAAAAAAAAAAAAAA250.10765189682642208No Hit
GCACTGTACAAAAAAAAAAAAAAAAAAAAAAAA240.10334582095336521No Hit
GTCATGAGCACTGTACGTACTCTGCGTTGATAC240.10334582095336521No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAT604.0017767E-1127.01
TATCATC604.0017767E-1127.02
TCGCAGA554.292815E-1027.07
ATCATCG554.292815E-1027.03
ATCGCAG554.292815E-1027.06
TCATCGC554.292815E-1027.04
CATCGCA554.292815E-1027.05
GATACCA756.4574124E-1021.627
CTCCATG450.001066311318.06
CCCCCAT450.001066311318.05
TATCAAC1400.017.3571431
GTACTAC552.0347317E-417.1818181
TGATACC1054.1980456E-815.42857126
ATCAACG1703.6379788E-1113.52
TCTGCGT7750.012.89032327
CCCCATG956.528973E-512.7894746
TCAACGC1705.493348E-1012.7058833
TCCATGA1001.0552643E-412.157
CAACGCA1801.3551471E-912.04
CGCATGA800.003470559111.81257
Produced by FastQC (version 0.11.2)