FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate8_7.35200000060600.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate8_7.35200000060600.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29285
Sequences flagged as poor quality0
Sequence length33
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTGCTGAAGACGTACTCTGCGTTGATACCA1980.6761140515622331No Hit
GCTGAAGACGTACTCAAAAAAAAAAAAAAAAAA1970.6726993341301007No Hit
TATCAACGCAGAGTGAATGGGCATTGCTGAAGA1450.4951340276592112No Hit
GTGAATGGGCATTGCTGAAGAAAAAAAAAAAAA1050.35854533037391156No Hit
GCTGAAGACGTACAAAAAAAAAAAAAAAAAAAA1000.34147174321324913No Hit
GTGAATGGGCATTGCTGAAAAAAAAAAAAAAAA970.33122759091685167No Hit
GCTGAAGACAAAAAAAAAAAAAAAAAAAAAAAA940.32098343862045414No Hit
ACGCAGAGTGAATGGGCATTGCTGAAGACGTAC760.25951852484206933No Hit
GTATCAACGCAGAGTGAATGGGCATTGCTGAAG760.25951852484206933No Hit
ACGCAGAGTGAATGGGCATTGCTGAAGAAAAAA670.22878606795287687No Hit
GTGAATGGGCATTGCTGAAGACAAAAAAAAAAA650.22195663308861194No Hit
TATCAACGCAGAGTGAATGGGCATTGCTGAAAA640.21854191565647943No Hit
ACGCAGAGTGAATGGGCATTGCTGAAAAAAAAA620.21171248079221444No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA500.17073587160662457No Hit
AACGCAGAGTGAATGGGCATTGCTGAAGAAAAA460.1570770018780946No Hit
GGTATCAACGCAGAGTGAATGGGCATTGCTGAA450.1536622844459621No Hit
GTATCATCGCAGAGTGAATGGGCATTGCTGAAG450.1536622844459621No Hit
GAGTACGTCTTCAGCAAAAAAAAAAAAAAAAAA440.1502475670138296No Hit
GCATTGCTGAAGACAAAAAAAAAAAAAAAAAAA440.1502475670138296No Hit
GAGTGAATGGGCATTGCTGAAAAAAAAAAAAAA430.14683284958169712No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA390.13317397985316715No Hit
GACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA390.13317397985316715No Hit
GTCTTCAGCAAAAAAAAAAAAAAAAAAAAAAAA360.12292982755676966No Hit
AACGCAGAGTGAATGGGCATTGCTGAAGACGTA360.12292982755676966No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT350.1195151101246372No Hit
GAGTGAATGGGCATTGCTGAAGAAAAAAAAAAA350.1195151101246372No Hit
GCATTGCTGAAGAAAAAAAAAAAAAAAAAAAAA350.1195151101246372No Hit
GTGAATGGGCATTGCTGAAGACGTACAAAAAAA340.1161003926925047No Hit
ACGCAGAGTGAATGGGCATTGCTGAAGACAAAA330.11268567526037221No Hit
GCTGAAGACGTAAAAAAAAAAAAAAAAAAAAAA330.11268567526037221No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA330.11268567526037221No Hit
GTGAATGGGGCATTGCTGAAAAAAAAAAAAAAA320.10927095782823971No Hit
TATCAACGCAGAGTGAATGGGGCATTGCTGAAG320.10927095782823971No Hit
GTGAATGGGCATTGCTGAAGACGTACTCAAAAA320.10927095782823971No Hit
GTGAATGGGGCATTGCTGAAGAAAAAAAAAAAA300.10244152296397474No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCAT200.00849181926.9897276
GGTATCA351.9787511E-423.134051
AACGCAT300.002217478122.4914386
CAACTCA300.002217478122.4914381
TACGCAG300.002217478122.4914385
TATCTAC300.002217478122.4914381
CTACGCA300.002217478122.4914384
AACTCAG350.00541930719.2783762
ACTCAGA350.00541930719.2783763
CTCAGAG350.00541930719.2783764
CATCGCA350.00541930719.2783765
GCGCATT901.2943201E-716.4937217
AAGCATT604.0079837E-415.7440067
GTATCAA1004.2866486E-714.844351
TCTGCGT7600.014.58522127
GTACTCA750.002168165812.59520510
GCGTTGA1851.5825208E-1012.42195627
TATCAAC1552.9278453E-812.188911
CTCTGCG9200.012.04866126
ACTCAAA906.1435485E-411.99543412
Produced by FastQC (version 0.11.2)