FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate8_47.35200000060b0b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate8_47.35200000060b0b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36608
Sequences flagged as poor quality0
Sequence length33
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTACGTTAGCAAAAAAAAAAAAAAAAAAAAA1010.275895979020979No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA850.2321896853146853No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA740.20214160839160839No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT720.1966783216783217No Hit
CCTCCAGTCTGCTAACGTACTCTGCGTTGATAC610.16663024475524474No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA520.14204545454545456No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA510.1393138111888112No Hit
CTCCAGTCTGCTAACGTACTCTGCGTTGATACC490.13385052447552448No Hit
GTCCAGTCTGCTAACGTACTCTGCGTTGATACC480.13111888111888112No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA460.1256555944055944No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA430.11746066433566432No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA420.11472902097902098No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA410.11199737762237763No Hit
GTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA380.10380244755244755No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA370.10107080419580419No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGATAC608.5734064E-720.2527
TGCGTTG4150.018.5421727
ATCAACG1001.2605597E-917.5500012
TATCAAC1102.382876E-1017.1818181
TCAACGC1154.474714E-1016.4347843
CAACGCA1258.185452E-1116.1999994
GTTGATA604.0311477E-415.7526
AGTACGT801.333655E-515.18752
GAGTACG852.3657443E-514.2941171
TCTGCGT13950.013.74193627
CCTTCCA802.2896068E-413.56
CACTCCA600.007368879413.56
CCCCTCC600.007368879413.55
GCTCCAG1253.7399695E-712.9599997
ACTCCAG1902.0008883E-1112.7894747
AACGCAG1603.3833203E-912.656255
CTCTGCG16000.011.89687526
CATCCAG1502.6253838E-711.7000017
ATTCCAG959.745303E-411.3684227
ACTCTGC17000.011.11764725
Produced by FastQC (version 0.11.2)