FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate8_46.35200000060ae0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate8_46.35200000060ae0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70036
Sequences flagged as poor quality0
Sequence length33
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTTGCAGTAACGTACTCTGCGTTGATACCAC2160.30841281626592043No Hit
GAGTACGTTACTGCAAAAAAAAAAAAAAAAAAA1930.2755725626820492No Hit
GTACGTTACTGCAAAAAAAAAAAAAAAAAAAAA1850.26414986578331145No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1140.16277343080701354No Hit
CCTCCTTGCAGTAACGTACTCTGCGTTGATACC930.13278885144782682No Hit
GTGAATGGGGAGTACGTTACTGCAAAAAAAAAA730.10423210920098235No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAT505.1077222E-926.981431
CCCCAAC200.00858497426.9814281
TATCATC551.39843905E-824.5461042
ATCTACG451.6595914E-624.0006352
TCTACGC451.6595914E-624.0006353
TACGCAG451.6595914E-624.0006355
TATCTAC451.6699705E-623.9834921
CTCAGAG401.8212202E-523.6256264
TCAGAGT352.0121693E-423.1434695
TGCGTTG7600.021.84926227
CTACGCA504.1445637E-621.6005734
AACTCAG454.522877E-521.0005552
ACTCAGA454.522877E-521.0005553
CAACTCA454.5479108E-520.9855561
TCGCAGA658.212919E-820.7697817
ATCGCAG658.212919E-820.7697816
CATCGCA658.212919E-820.7697815
AACCCTC404.9620034E-420.2505365
ATCATCG608.8058914E-720.2505363
TCATCGC701.7944876E-719.2862244
Produced by FastQC (version 0.11.2)