FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate8_45.35200000060ac6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate8_45.35200000060ac6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10262
Sequences flagged as poor quality0
Sequence length33
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA480.4677450789319821No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT470.45800038978756574No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA400.38978756577665175No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA300.29234067433248884No Hit
GTGAATGGGATAGCGTCAAAAAAAAAAAAAAAA270.2631066068992399No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA260.2533619177548236No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA240.23387253946599104No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA220.21438316117715844No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA210.20463847203274216No Hit
GTGAATGGGGTATCTCGACGCTAAAAAAAAAAA210.20463847203274216No Hit
GCCATGGGGAGATACCGTCTGTGATCCATGGAT210.20463847203274216No Hit
GGGAGATACCGTCTGTGATCCATGGATCTCCGA210.20463847203274216No Hit
GTGAATGGGTAGCGTCGAAAAAAAAAAAAAAAA190.18514909374390956No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA190.18514909374390956No Hit
GCATTGGATGGATGCCCGGGCATTGAGAAGGAA180.17540440459949327No Hit
CTCTTTGGACTTTGAAAACTTAGCGAACTGAAA170.165659715455077No Hit
GTGAATGGGTAGCGTCAAAAAAAAAAAAAAAAA170.165659715455077No Hit
CGTCGAGATACGTACTCTGCGTTGATACCACTG160.15591502631066068No Hit
CATCTAAGTAGCTAAAAAAAAAAAAAAAAAAAA160.15591502631066068No Hit
GTGAATGGGTAGCGTCGAGATAAAAAAAAAAAA150.14617033716624442No Hit
ACGCAGAGTGAATGGGTAGCGTCAAAAAAAAAA150.14617033716624442No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA140.1364256480218281No Hit
TATCAACGCAGAGTGAATGGGTAGCGTCGAGAT140.1364256480218281No Hit
CATCTAAGTAGCTAAAGGAAGGGAAATCAACCG130.1266809588774118No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA130.1266809588774118No Hit
ATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA130.1266809588774118No Hit
TACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA130.1266809588774118No Hit
GTGAATGGGTAGCGTCGAGAAAAAAAAAAAAAA130.1266809588774118No Hit
GTGAATGGGATAGCGTCGAGAAAAAAAAAAAAA120.11693626973299552No Hit
CCCTAGCGTCGAGATACGTACTCTGCGTTGATA110.10719158058857922No Hit
AGCGTCGAGATACGTACTCTGCGTTGATACCAC110.10719158058857922No Hit
GTGAATGGGGTAGCGTCAAAAAAAAAAAAAAAA110.10719158058857922No Hit
ACGCAGAGTGAATGGGTAGCGTCGAGAAAAAAA110.10719158058857922No Hit
TATCAACGCAGAGTGAATGGGATAGCGTCGAGA110.10719158058857922No Hit
ACGCAGAGTGAATGGGGTAGCGTCAAAAAAAAA110.10719158058857922No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGATA200.00822327327.08
GATACCG200.00822327327.011
GGGAGAT200.00822327327.07
ATACCGT200.00822327327.012
GGGGAGA200.00822327327.06
AGATACC200.00822327327.010
ATGGGTA351.8531654E-423.1428595
TGGGTAG300.002115517622.56
CATGGGG300.002115517622.53
AATGGGT404.5740596E-420.254
TGCGTTG802.7566784E-818.562527
TATCAAC603.6853598E-415.751
TCTGCGT2850.015.63157827
CTCTGCG3650.012.2054826
CTGCGTT1254.3706914E-611.87999926
CTAGCGT1851.5006663E-911.6756758
ACTCTGC4050.011.025
AGATACG4350.010.86206917
ACGTACT4550.010.68131821
ATACGTA4550.010.68131819
Produced by FastQC (version 0.11.2)