FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate8_44.35200000060aad.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate8_44.35200000060aad.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35287
Sequences flagged as poor quality0
Sequence length33
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAATGGGCAGAGTGGCAAAAAAAAAAAAAAA850.24088191118542238No Hit
GGCTAAACGTACTCAAAAAAAAAAAAAAAAAAA550.15586476606115565No Hit
ACGCAGAGTGAATGGGCAGAGTGGCAAAAAAAA530.15019695638620456No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC500.14169524187377788No Hit
TATCAACGCAGAGTGAATGGGCAGAGTGGCAAA490.13886133703630232No Hit
GTGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA460.13035962252387565No Hit
TATCAACGCAGAGTGAATGGGCTAAACGTACTC410.11619009833649786No Hit
AACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA390.11052228866154673No Hit
GTGAATGGGCAGAGTGGAAAAAAAAAAAAAAAA370.10485447898659563No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCATC401.7824528E-523.6349582
ATCATCG401.7824528E-523.6349583
TCATCGC454.4274475E-521.008854
GTATCAT509.95712E-518.9079671
CATCGCA501.00668745E-418.8811075
GTTGATA651.835142E-618.70018627
ATCGCAG552.0890178E-417.1646426
TATCAAC1307.2759576E-1216.6223871
GTATCAA500.002183704616.206831
TCTGCGT7000.016.0138927
TCGCAGA604.0565958E-415.7342587
ATCAACG1508.185452E-1114.4060712
CGCCAGA802.3052655E-413.48650556
AACAGAG853.8498716E-412.6931827
TCAACGC1501.8804712E-812.6053113
CGTTGAT1203.369847E-612.38021627
CTCTGCG9550.011.73792926
CTCAGAG959.811425E-411.3570577
GCCAGAG1659.089017E-710.6257317
TGCGTTG2305.4023985E-1010.56967127