FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate8_42.35200000060a69.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate8_42.35200000060a69.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22744
Sequences flagged as poor quality0
Sequence length33
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGTACTCTGCGTTGATACAAAAAAAAAAAAA1670.7342595849454802No Hit
AACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA1360.5979599015124868No Hit
TATCAACGCAGAGTGAATGGGAACGTACTCTGC1050.4616602180794935No Hit
GAAACGTACTCTGCGTAAAAAAAAAAAAAAAAA1040.4572634540977841No Hit
ACTCTGCGTTGATACAAAAAAAAAAAAAAAAAA900.3957087583538516No Hit
GTGAATGGGAACGTACTCTGCGTTGATACAAAA880.38691523039043263No Hit
TATCAACGCAGAGTGAATGGGAAACGTACTCTG670.29458318677453393No Hit
GCGTTGATACAAAAAAAAAAAAAAAAAAAAAAA640.28139289482940555No Hit
ACGCAGAGTGAATGGGAACGTACTCTGCGTTGA600.2638058389025677No Hit
GGAACGTACTCTGCGTTGATACAAAAAAAAAAA500.21983819908547308No Hit
GAAACGTACTCTGCGTTGATACAAAAAAAAAAA440.19345761519521631No Hit
AACGTACTCTGCGTTGATACCAAAAAAAAAAAA440.19345761519521631No Hit
GTATCAACGCAGAGTGAATGGGAACGTACTCTG430.18906085121350685No Hit
AACGTACTCTGCGTTAAAAAAAAAAAAAAAAAA420.1846640872317974No Hit
GTACTCTGCGTTGATACAAAAAAAAAAAAAAAA410.18026732325008793No Hit
AACGTACTCTGCGTTGAAAAAAAAAAAAAAAAA400.17587055926837847No Hit
GAGTGAATGGGAACGTACTCTGCGTTGATACAA400.17587055926837847No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA390.171473795286669No Hit
GTGAATGGGCAGTCTCTGGAAAAAAAAAAAAAA350.15388673935983116No Hit
GTGAATGGGCAGTCTCAAAAAAAAAAAAAAAAA340.1494899753781217No Hit
TATCAACGCAGAGTGAATGGGCAGTCTCTGGAA320.14069644741470277No Hit
GAGTACGTTTCCAAAAAAAAAAAAAAAAAAAAA320.14069644741470277No Hit
AACGCAGAGTGAATGGGAACGTACTCTGCGTTG310.1362996834329933No Hit
GAACGTACTCTGCGTTGATACAAAAAAAAAAAA290.1275061554695744No Hit
AACGTACTCTGCGAAAAAAAAAAAAAAAAAAAA290.1275061554695744No Hit
GTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA280.12310939148786493No Hit
ACTCTGCGTTGATACCAAAAAAAAAAAAAAAAA280.12310939148786493No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA270.11871262750615547No Hit
GGTATCAACGCAGAGTGAATGGGAACGTACTCT260.11431586352444602No Hit
ACGCAGAGTGAATGGGCAGTCTCTGGAAAAAAA260.11431586352444602No Hit
GTATCAACGCAGAGTGAATGGGAAACGTACTCT260.11431586352444602No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT250.10991909954273654No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA250.10991909954273654No Hit
GTCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAA240.1055223355610271No Hit
GCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA240.1055223355610271No Hit
GTATCATCGCAGAGTGAATGGGAACGTACTCTG230.10112557157931762No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA230.10112557157931762No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAT306.862495E-526.9977951
TACGCAG306.862495E-526.9977955
CTACGCA306.862495E-526.9977954
ATCTACG257.595748E-426.9977932
TCTACGC257.595748E-426.9977933
TATCTAC257.595748E-426.9977931
TATCATC351.9556521E-423.1409662
TCGCAGA351.9556521E-423.1409667
ATCATCG351.9556521E-423.1409663
TCATCGC351.9556521E-423.1409664
CATCGCA351.9556521E-423.1409665
ATCGCAG404.8241808E-420.2483446
TATCAAC1154.1472958E-1016.433441
AACAGTC500.002158684416.1986777
ATCAACG1202.1495907E-713.4988982
TCTGCGT8200.012.70383627
GCCCAGT750.00213724712.5989696
TCAACGC1358.99352E-711.9990193
CAACGCA1401.3956906E-611.5704834
TCAGTCT2151.4915713E-1011.3014028
Produced by FastQC (version 0.11.2)