FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate8_40.35200000060a26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate8_40.35200000060a26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29526
Sequences flagged as poor quality0
Sequence length33
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCAAGCTCAACACGTACTCTGCGTTGATACCA2090.7078507078507079No Hit
GCTCAACACGTACTCAAAAAAAAAAAAAAAAAA1730.5859242701347964No Hit
GTGAATGGGTCAAGCTCAAAAAAAAAAAAAAAA1000.338684549210865No Hit
TATCAACGCAGAGTGAATGGGTCAAGCTCAAAA450.15240804714488923No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT440.1490212016527806No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA440.1490212016527806No Hit
ACGCAGAGTGAATGGGTCAAGCTCAAAAAAAAA430.14563435616067197No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC410.13886066517645465No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA410.13886066517645465No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA400.135473819684346No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA360.12192643771591138No Hit
CGTCAAGCTCAACACGTACTCTGCGTTGATACC330.11176590123958545No Hit
GTGAATGGGTCAAGCAAAAAAAAAAAAAAAAAA320.1083790557474768No Hit
CCGTCAAGCTCAACACGTACTCTGCGTTGATAC320.1083790557474768No Hit
GGTCAAGCTCAACACGTACTCTGCGTTGATACC300.1016053647632595No Hit
GTCAAGCTCAAAAAAAAAAAAAAAAAAAAAAAA300.1016053647632595No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTCAA553.661953E-722.090917
TATCAAC901.2732926E-1121.01
ATCAACG1052.3501343E-916.7142852
TCTGCGT3700.014.95945927
TCAACGC1252.3583198E-814.043
CAACGCA1303.9486622E-813.5000014
CTCTGCG4350.012.72413826
ACTCTGC4450.012.43820325
CGTCAAG2700.012.08
AACGCAG1502.5589907E-711.7000015
GTACTCT4750.011.65263123
TACTCTG4750.011.65263124
TGTCAAG959.6475164E-411.3684228
CGTACTC5000.010.822
ACGTACT5050.010.69306921
TCAAGCT5850.010.61538410
CAAGCTC5850.010.61538411
AAGCTCA5750.010.33043512
TGCGTTG1050.00222465210.28571427
AGCTCAA5800.010.2413813
Produced by FastQC (version 0.11.2)