FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate8_37.352000000609d4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate8_37.352000000609d4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23646
Sequences flagged as poor quality0
Sequence length33
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTAGAGAAAAAAAAAAAAAAAAAAAAAAAA2901.2264230736699653No Hit
GTACTAGAGACGTACTCAAAAAAAAAAAAAAAA1690.7147086187938763No Hit
CTCTAGTACCGAAAAAAAAAAAAAAAAAAAAAA1540.6512729425695678No Hit
CGGTACTAGAGACGTACTCTGCGTTGATACCAC1270.5370887253658124No Hit
GTACTAGAGACAAAAAAAAAAAAAAAAAAAAAA1070.45250782373340104No Hit
GTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA790.334094561448025No Hit
GTACTAGAGACGTAAAAAAAAAAAAAAAAAAAA770.3256364712847839No Hit
GTACTAGAGACGAAAAAAAAAAAAAAAAAAAAA450.19030702867292568No Hit
GTACTAGAAAAAAAAAAAAAAAAAAAAAAAAAA450.19030702867292568No Hit
CCTCGGTACTAGAGACGTACTCTGCGTTGATAC430.18184893850968453No Hit
GTACTAGAGACGTACAAAAAAAAAAAAAAAAAA410.1733908483464434No Hit
GTGAATGGGTCGGTACAAAAAAAAAAAAAAAAA410.1733908483464434No Hit
CTAGAGACGTACTCAAAAAAAAAAAAAAAAAAA370.1564746680199611No Hit
TATCAACGCAGAGTGAATGGGTACTAGAGACGT320.13532944261185822No Hit
GTGAATGGGTCGGTACTAGAGAAAAAAAAAAAA290.12264230736699652No Hit
CTCGGTACTAGAGACGTACTCTGCGTTGATACC250.10572612704051426No Hit
GTACTAAAAAAAAAAAAAAAAAAAAAAAAAAAA250.10572612704051426No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCGGT350.00537648319.2857157
TATCAAC803.268906E-818.56251
ACCGAAA450.001066874718.0000028
CCGAAAA500.002160780416.29
TCAACGC851.2810015E-615.8823523
ATCAACG851.2810015E-615.8823522
CAACGCA902.3240846E-615.0000014
TCTGCGT3000.014.427
TAGTACC1151.2838791E-714.0869574
TACCGAA700.001272666313.57
TCTAGTA1051.1471273E-512.8571422
GTACCGA1051.1471273E-512.8571426
AGTACCG1051.1471273E-512.8571425
CTAGTAC1051.1471273E-512.8571423
CTCTAGT1152.9208893E-511.7391311
AACGCAG959.5192617E-411.3684225
CTCTGCG3700.011.31081126
ACGCAGA1903.992136E-810.6578951
ACTCTGC4100.010.53658525
AGTGAAT3201.8189894E-129.7031252
Produced by FastQC (version 0.11.2)