FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate8_32.35200000060934.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate8_32.35200000060934.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20524
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATCCACGTACTCAAAAAAAAAAAAAAAAAAA4041.9684272071720914No Hit
TATCAACGCAGAGTGAATGGGCTAACTATCCAC2991.4568310270902358No Hit
GTGAATGGGCTAACTATCCACAAAAAAAAAAAA1940.9452348470083805No Hit
GGCTAACTATCCACGTACTCTGCGTTGATACCA1930.9403625024361724No Hit
ACGCAGAGTGAATGGGCTAACTATCCACAAAAA1540.7503410641200546No Hit
ATCCACGTACTCAAAAAAAAAAAAAAAAAAAAA1430.6967452738257649No Hit
GGCTAACTATCCACAAAAAAAAAAAAAAAAAAA1250.6090430715260183No Hit
GTATCAACGCAGAGTGAATGGGCTAACTATCCA1210.5895536932371858No Hit
GTGAATGGGCTAACTATCCACGTACTCAAAAAA1180.5749366595205613No Hit
CTATCCACAAAAAAAAAAAAAAAAAAAAAAAAA1140.5554472812317287No Hit
TATCAACGCAGAGTGAATGGGGCTAACTATCCA980.47748976807639837No Hit
GAGTGAATGGGCTAACTATCCACAAAAAAAAAA940.45800038978756574No Hit
GGCTAACTATCCACGTACTCAAAAAAAAAAAAA890.43363866692652503No Hit
ACGCAGAGTGAATGGGCTAACTATCCACGTACT850.4141492886376924No Hit
ATCCACAAAAAAAAAAAAAAAAAAAAAAAAAAA750.365425842915611No Hit
AACGCAGAGTGAATGGGCTAACTATCCACGTAC690.3361917754823621No Hit
GTGAATGGGGCTAACTATCCACGTACTCAAAAA660.3215747417657377No Hit
CTATCCACGTACAAAAAAAAAAAAAAAAAAAAA640.31183005262132135No Hit
AACGCAGAGTGAATGGGCTAACTATCCACAAAA590.28746832976028064No Hit
ACTATCCACAAAAAAAAAAAAAAAAAAAAAAAA570.27772364061586435No Hit
TATCTACGCAGAGTGAATGGGCTAACTATCCAC540.2631066068992399No Hit
GTGAATGGGCTAACTATCCAAAAAAAAAAAAAA530.2582342623270318No Hit
ACGCAGAGTGAATGGGCTAACTATCCACGTACA530.2582342623270318No Hit
ACTATCCACGTACTCAAAAAAAAAAAAAAAAAA530.2582342623270318No Hit
GTGAATGGGCTAACTAAAAAAAAAAAAAAAAAA520.2533619177548236No Hit
GTGAATGGGCTAACTATCCACGTACAAAAAAAA510.24848957318261547No Hit
GTGAATGGGGCTAACTATCCACAAAAAAAAAAA500.24361722861040735No Hit
GTGAATGGGCTAACTATAAAAAAAAAAAAAAAA500.24361722861040735No Hit
CGTACTCAAAAAAAAAAAAAAAAAAAAAAAAAA480.23387253946599104No Hit
GCTAACTATCCACGTACTCTGCGTTGATACCAC480.23387253946599104No Hit
GGTATCAACGCAGAGTGAATGGGCTAACTATCC470.22900019489378287No Hit
CTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAA450.21925550574936659No Hit
ACGCAGAGTGAATGGGGCTAACTATCCACGTAC440.21438316117715844No Hit
GAGTGAATGGGGCTAACTATCCACGTACTCAAA440.21438316117715844No Hit
ATCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA440.21438316117715844No Hit
GTATCAACGCAGAGTGAATGGGGCTAACTATCC430.20951081660495033No Hit
ATCCACGTACAAAAAAAAAAAAAAAAAAAAAAA430.20951081660495033No Hit
TATCAACGCAGAGTGAATGGGCTAACTATCCAA420.20463847203274216No Hit
GAGTGAATGGGCTAACTATCCACGTACTCAAAA380.18514909374390956No Hit
GTATCATCGCAGAGTGAATGGGCTAACTATCCA370.18027674917170142No Hit
GGGCTAACTATCCACGTACTCAAAAAAAAAAAA360.17540440459949327No Hit
GTGAATGGGGCTAACTAAAAAAAAAAAAAAAAA360.17540440459949327No Hit
GGCTAACTATCCACGTACAAAAAAAAAAAAAAA340.165659715455077No Hit
ACGCAGAGTGAATGGGCTAACTATCCAAAAAAA320.15591502631066068No Hit
GCTAACTATCCACGTACTCAAAAAAAAAAAAAA320.15591502631066068No Hit
CTATCCAAAAAAAAAAAAAAAAAAAAAAAAAAA320.15591502631066068No Hit
CGGCTAACTATCCACGTACTCTGCGTTGATACC310.15104268173845253No Hit
TCCACGTACTCAAAAAAAAAAAAAAAAAAAAAA300.14617033716624442No Hit
ATCAACGCAGAGTGAATGGGCTAACTATCCACG300.14617033716624442No Hit
GAATGGGGCTAACTATCCACGTACTCAAAAAAA300.14617033716624442No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA290.14129799259403625No Hit
GAGTGAATGGGGCTAACTATCCACAAAAAAAAA290.14129799259403625No Hit
GGGCTAACTATCCACGTACTCTGCGTTGATACC280.1364256480218281No Hit
ATCAACGCAGAGTGAATGGGCTAACTATCCACA280.1364256480218281No Hit
CGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAA280.1364256480218281No Hit
TATCAACGCAGAGTGAATGGGCTAACTATAAAA270.13155330344961996No Hit
TATCAACGCAGAGTGAATGGGCTAACTAAAAAA260.1266809588774118No Hit
TATCTACGCAGAGTGAATGGGGCTAACTATCCA260.1266809588774118No Hit
GAGTGAATGGGCTAACTATCCACGTACAAAAAA260.1266809588774118No Hit
GGCTAACTATCCAAAAAAAAAAAAAAAAAAAAA250.12180861430520368No Hit
CACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA250.12180861430520368No Hit
GAGTGAATGGGGCTAACTATCCACGTACAAAAA250.12180861430520368No Hit
GAGTGAATGGGCTAACTATAAAAAAAAAAAAAA240.11693626973299552No Hit
GGGAGATACCGTCTGTGATCCATGGATCTCCGA240.11693626973299552No Hit
GAGTGAATGGGCTAACTATCCAAAAAAAAAAAA240.11693626973299552No Hit
GTGAATGGGGCTAACTATCCACGTACAAAAAAA230.11206392516078738No Hit
GAGTGAATGGGGCTAACTAAAAAAAAAAAAAAA230.11206392516078738No Hit
CAACGCAGAGTGAATGGGCTAACTATCCACGTA230.11206392516078738No Hit
ACGCAGAGTGAATGGGCTAACTAAAAAAAAAAA230.11206392516078738No Hit
GTGAATGGGGCTAACAAAAAAAAAAAAAAAAAA210.10231923601637108No Hit
GTATCATCGCAGAGTGAATGGGGCTAACTATCC210.10231923601637108No Hit
ACGCAGAGTGAATGGGCTAACTATAAAAAAAAA210.10231923601637108No Hit
GAGTGAATGGGGCTAACTATAAAAAAAAAAAAA210.10231923601637108No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAT257.5672165E-426.9999981
TCTACGC356.178927E-626.9999983
TACGCAG356.178927E-626.9999985
TATCATC257.5672165E-426.9999982
CTACGCA356.178927E-626.9999984
ATCTACG401.7412638E-523.6252
TATCTAC401.7412638E-523.6251
TATCAAC1350.020.01
TCGCAGA350.005353392619.2857137
ATCATCG350.005353392619.2857133
ATCGCAG350.005353392619.2857136
TCATCGC350.005353392619.2857134
CATCGCA350.005353392619.2857135
GTATCAA450.001060509418.0000021
ATCAACG1450.017.6896552
TCAACGC1550.016.5483883
TGCGTTG2350.015.51063827
TCGGCTA707.100167E-515.428577
CAACGCA2003.6379788E-1212.8254
TCTGCGT6150.011.63414627
Produced by FastQC (version 0.11.2)