FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate8_31.3520000006091a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate8_31.3520000006091a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29826
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTCAGACATCGCAAAAAAAAAAAAAAAAAAA1080.3621001810500905No Hit
GAGTACGTCAGACATCGCAAAAAAAAAAAAAAA980.32857238650841547No Hit
GTGAATGGGATGTCTGACGTACTCAAAAAAAAA830.2782806946959029No Hit
GTACGTCAGACATCGCAAAAAAAAAAAAAAAAA640.2145778850667203No Hit
GTGAATGGGGACATCGCAAAAAAAAAAAAAAAA590.1978139877958828No Hit
GTGAATGGGACATCGCAAAAAAAAAAAAAAAAA590.1978139877958828No Hit
GTCTGACGTACTCAAAAAAAAAAAAAAAAAAAA560.18775564943338027No Hit
TATCAACGCAGAGTGAATGGGATGTCTGACGTA540.18105009052504525No Hit
TGTCTGACGTACTCAAAAAAAAAAAAAAAAAAA480.16093341380004023No Hit
GGGTCAGACATCGCAAAAAAAAAAAAAAAAAAA440.14752229598337022No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA400.1341111781667002No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA380.1274056192583652No Hit
GTCAGACATCGCAAAAAAAAAAAAAAAAAAAAA350.11734728089586267No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA340.11399450144169516No Hit
ACGCAGAGTGAATGGGATGTCTGACGTACTCAA340.11399450144169516No Hit
GTGAATGGGGAGTACGTCAGACATCGCAAAAAA330.11064172198752766No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT320.10728894253336015No Hit
GACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA310.10393616307919265No Hit
GAGTGAATGGGATGTCTGACGTACTCAAAAAAA310.10393616307919265No Hit
CTGACGTACTCAAAAAAAAAAAAAAAAAAAAAA310.10393616307919265No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTCAG200.00847883127.02
ATCTACG401.7763723E-523.6252
TCTACGC401.7763723E-523.6253
TATCTAC401.7763723E-523.6251
CTACGCA401.7763723E-523.6254
TACGCAG454.4125678E-521.05
TATCAAC1151.8189894E-1218.7826081
ATCAACG1454.5474735E-1114.8965522
TGCGTTG1302.4720066E-914.53846227
TCAACGC1601.4551915E-1114.343753
TCTGCGT4150.014.31325327
GTATCAA750.002163794812.5999991
CAACGCA1851.6370905E-1012.4054054
AACGCAG2101.07320375E-1011.5714285
ATGGGAC850.005521178211.1176475
CTGCGTT1956.123082E-810.38461627
CCTTGCG1503.9745973E-59.98
CTCTGCG6100.09.73770526
ACGCAGA3355.456968E-129.2686576
CGCAGAG3355.456968E-129.2686577
Produced by FastQC (version 0.11.2)