FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate8_29.352000000608d5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate8_29.352000000608d5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40811
Sequences flagged as poor quality0
Sequence length33
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTGGACCTTACGTACTCTGCGTTGATACCA3970.9727769473916346No Hit
GCTTGGACCTTACGTACTCTGCGTTGATACCAC3040.7448972090857857No Hit
GCCTTACGTACTCAAAAAAAAAAAAAAAAAAAA2150.526818749846855No Hit
GAGTACGTAAGGTCCAAAAAAAAAAAAAAAAAA1830.44840851731150916No Hit
GTACGTAAGGTCCAAAAAAAAAAAAAAAAAAAA1490.36509764524270416No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC1370.3356938080419495No Hit
TATCAACGCAGAGTGAATGGGCCTTACGTACTC1160.28423709294062877No Hit
TACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA1020.24993261620641494No Hit
GACCTTACGTACTCAAAAAAAAAAAAAAAAAAA970.23768101737276717No Hit
ACGCAGAGTGAATGGGCCTTACGTACTCAAAAA880.2156281394722011No Hit
GGCCTTACGTACTCAAAAAAAAAAAAAAAAAAA810.19847590110509422No Hit
GTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA700.1715223836710691No Hit
ACCTTACGTACTCAAAAAAAAAAAAAAAAAAAA690.16907206390433951No Hit
CCTTACGTACTCAAAAAAAAAAAAAAAAAAAAA680.16662174413760994No Hit
GTGAATGGGGAGTACGTAAGGTCCAAAAAAAAA680.16662174413760994No Hit
GTGAATGGGCCTTACGTACTCAAAAAAAAAAAA670.16417142437088042No Hit
TATCAACGCAGAGTGAATGGGACCTTACGTACT650.15927078483742127No Hit
GTGAATGGGAGTACGTAAGGTCCAAAAAAAAAA610.14946950577050305No Hit
GTGAATGGGACCTTACGTACTCAAAAAAAAAAA600.14701918600377348No Hit
GTGAATGGGAGGTCCAAAAAAAAAAAAAAAAAA540.13231726740339617No Hit
TACGTACTCTGCGTTGATACAAAAAAAAAAAAA520.12741662786993702No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA510.12496630810320747No Hit
GTATCAACGCAGAGTGAATGGGCCTTACGTACT500.12251598833647791No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGC500.12251598833647791No Hit
GCTAAATACGGGCGAGAGACCGATAGCGAACAA490.12006566856974835No Hit
GTGAATGGGGGTCCAAAAAAAAAAAAAAAAAAA490.12006566856974835No Hit
GAATGGGACCTTACGTACTCAAAAAAAAAAAAA480.1176153488030188No Hit
GGCTAAATACGGGCGAGAGACCGATAGCGAACA480.1176153488030188No Hit
GTGAATGGGTACGTAAGGTCCAAAAAAAAAAAA450.11026438950283012No Hit
GGGTACGTAAGGTCCAAAAAAAAAAAAAAAAAA430.105363749969371No Hit
GTATCATCGCAGAGTGAATGGGCCTTACGTACT430.105363749969371No Hit
AACAAGTACCGCGAGGTAAAAAAAAAAAAAAAA420.10291343020264145No Hit
AACGCAGAGTGAATGGGCCTTACGTACTCAAAA410.1004631104359119No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCAG257.7392627E-426.975465
ATCTACG300.002223454922.5071682
TCTACGC300.002223454922.5071683
TATCTAC300.002223454922.5071681
CTACGCA300.002223454922.5071684
TGGGATG350.00543355419.29185718
GATTGCT350.005472365319.2681876
TATCAAC1500.018.906021
ATCAACG1450.018.6266212
TCAACGC1500.017.1054463
CAACGCA1550.016.5536584
AACTGCT550.00417363314.7138876
AACGCAG1950.013.833575
GATACCA1051.193903E-512.86123827
ATGGGAG750.002202726212.5885485
TGATACC1101.9263232E-512.27663726
TCTGCGT10700.011.73738227
TGCGTTG4400.011.66280527
TTGATAC1452.794993E-510.24464225
CTTGCTT1205.9852266E-410.1157988
Produced by FastQC (version 0.11.2)