FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate8_28.352000000608bb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate8_28.352000000608bb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20103
Sequences flagged as poor quality0
Sequence length33
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTCCTCTACGTACTCTGCGTTGATACCA1890.9401581853454709No Hit
CTCGTCCTCTACGTACTCTGCGTTGATACCACT1160.5770283042331991No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA800.39795055464358553No Hit
GTGAATGGGATCTCGTCCAAAAAAAAAAAAAAA760.37805302691140624No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA550.2735910063174651No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA470.2337959508531065No Hit
CATCTCGTCCTCTACGTACTCTGCGTTGATACC460.2288215689200617No Hit
GTGAATGGGATCTCGTCAAAAAAAAAAAAAAAA460.2288215689200617No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT440.21887280505397205No Hit
TATCAACGCAGAGTGAATGGGATCTCGTCCAAA380.18902651345570312No Hit
CCCCCATCTCGTCCTCTACGTACTCTGCGTTGA340.16912898572352383No Hit
ACGCAGAGTGAATGGGATCTCGTCCAAAAAAAA310.1542058399243894No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA310.1542058399243894No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA310.1542058399243894No Hit
GAGTGAATGGGATCTCGTCCAAAAAAAAAAAAA300.14923145799134457No Hit
GTGAATGGGATCTCGTCCTCAAAAAAAAAAAAA290.14425707605829977No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA290.14425707605829977No Hit
GATCTCGTCCAAAAAAAAAAAAAAAAAAAAAAA250.12435954832612048No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA240.11938516639307566No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA220.10943640252698603No Hit
AACGCAGAGTGAATGGGATCTCGTCCAAAAAAA220.10943640252698603No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA210.1044620205939412No Hit
ATCTCGTCCTCAAAAAAAAAAAAAAAAAAAAAA210.1044620205939412No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACCA703.5805115E-617.35714327
TATCAAC806.6751454E-716.8751
CTATCTC550.004051567514.7272735
ATCAACG954.0042814E-614.2105262
CAACGCA1057.083363E-714.1428574
GCGTTGA2300.014.08695627
TGATACC802.209618E-413.526
TCAACGC1006.811895E-613.4999993
TTGATAC853.6913584E-412.70588125
GTTGATA959.41362E-411.36842224
TGCGTTG3700.010.21621627
CGTTGAT1150.00461561659.39130423
AACGCAG1300.00118028779.3461535
CTGCGTT4150.09.10843426
TCTGCGT6450.09.027
TATCTCG1555.8858696E-48.7096775
CCATCTC1555.8858696E-48.7096777
AATCTCG2051.3220502E-58.5609768
GCAGAGT1752.048653E-48.4857158
CAGAGTG1700.00147335377.9411769
Produced by FastQC (version 0.11.2)