FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate8_27.35200000060892.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate8_27.35200000060892.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12782
Sequences flagged as poor quality0
Sequence length33
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA470.36770458457205446No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA460.359881082772649No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT430.33641057737443275No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA380.2972930683774057No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA370.2894695665780003No Hit
GAGTACGTTTCGACCAAAAAAAAAAAAAAAAAA340.26599906117978406No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA310.24252855578156784No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA270.2112345485839462No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA250.19558754498513534No Hit
GTGAATGGGTCGTTGGTCAAAAAAAAAAAAAAA240.18776404318572992No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA230.1799405413863245No Hit
TCGTTGGTCGAAACGTACTCTGCGTTGATACCA230.1799405413863245No Hit
GTGAATGGGTCGTTGGTAAAAAAAAAAAAAAAA220.17211703958691912No Hit
GTTTCGACCAAAAAAAAAAAAAAAAAAAAAAAA220.17211703958691912No Hit
GTGAATGGGTCGTTGGAAAAAAAAAAAAAAAAA210.16429353778751368No Hit
GTCGTTGGTCGAAACGTACTCTGCGTTGATACC200.1564700359881083No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA190.14864653418870286No Hit
GTGAATGGGGAGTACGTTTCGACCAAAAAAAAA190.14864653418870286No Hit
CGTTTCGACCAAAAAAAAAAAAAAAAAAAAAAA180.14082303238929744No Hit
GTGAATGGGGTACGTTTCGACCAAAAAAAAAAA180.14082303238929744No Hit
TATCAACGCAGAGTGAATGGGTCGTTGGTCAAA170.13299953058989203No Hit
GAGTGAATGGGGTACGTTTCGACCAAAAAAAAA160.12517602879048662No Hit
TATCAACGCAGAGTGAATGGGTCGAAACGTACT160.12517602879048662No Hit
AACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA150.11735252699108122No Hit
GAGTGAATGGGGTCGTTGGAAAAAAAAAAAAAA140.10952902519167579No Hit
GTGAATGGGTACGTTTCGACCAAAAAAAAAAAA130.10170552339227039No Hit
GGTATCAACGCAGAGTGAATGGGAAGCAGTGGT130.10170552339227039No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTACG200.00830043527.02
TCTACGC200.00830043527.03
TATCTAC200.00830043527.01
TATCAAC1059.640644E-1118.01
GTATCAA551.94473E-417.1818181
GCGTTGA653.585525E-516.61538527
TCTGCGT3500.016.58571427
CTCTGCG4200.013.82142826
TCAACGC1405.689799E-913.53
ATCAACG1405.689799E-913.52
CAACGCA1459.322321E-913.0344824
ACTCTGC4500.012.90000125
TACTCTG4650.012.48387124
AAACGTA5050.012.02970419
AATGGGT905.713621E-412.04
GAAACGT5000.011.87999918
AACGCAG1603.7000063E-811.81255
CGAAACG5050.011.76237717
TGCGTTG1152.6802076E-511.73913127
GTACTCT4950.011.72727223
Produced by FastQC (version 0.11.2)