FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate8_25.3520000006085f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate8_25.3520000006085f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13263
Sequences flagged as poor quality0
Sequence length33
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTGGATGGATGCCCGGGCATTGAGAAGGAA1621.221443112418005No Hit
GGGAGATACCGTCTGTGATCCATGGATCTCCGA1511.1385056171303627No Hit
CATCTAAGTAGCTAAAGGAAGGGAAATCAACCG1050.7916760913820403No Hit
GCCATGGGGAGATACCGTCTGTGATCCATGGAT920.6936590514966449No Hit
TGGCTAGTCTGCGCTCTTTGGACTTTGAAAACT740.5579431501168665No Hit
CTCTTTGGACTTTGAAAACTTAGCGAACTGAAA700.5277840609213602No Hit
GCTAAAGGAAGGGAAATCAACCGAGACCCCGTT650.49008519942697726No Hit
CATCTAAGTAGCTAAAAAAAAAAAAAAAAAAAA640.4825454271281007No Hit
CGTTAGTAGCGGCGAGCGAGAGCGGATTTGGGA530.39960793184045845No Hit
TCCAAGCTCCGTGGCTAGTCTGCGCTCTTTGGA530.39960793184045845No Hit
CAACGAACCAAGACGTACTCTGCGTTGATACCA520.39206815954158186No Hit
TATCCAAGCTCCGTGGCTAGTCTGCGCTCTTTG490.36944884264495215No Hit
GAGTAGGGGTCGCGAGAGAGCAGAGCGTACCGC490.36944884264495215No Hit
CTTTGGACTTTGAAAACTTAGCGAACTGAAACA490.36944884264495215No Hit
GTGAATGGGGAATGCATTGGATGGATGCCCGGG480.36190907034607556No Hit
GATTTGGGATTTTAAAAAAAAAAAAAAAAAAAA460.34682952574832243No Hit
GGCGAAAGGCCATGGGGAGATACCGTCTGTGAT400.30159089195506295No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA370.27897157505843323No Hit
GAGAAGGAAGGACGCTTTCAGAGGCGAAAGGCC360.2714318027595567No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC350.2638920304606801No Hit
TCTGTGATCCATGGATCTCCGATCGGGAAACCG350.2638920304606801No Hit
GAGATACCGTCTGTGATCCATGGATCTCCGATC350.2638920304606801No Hit
AGCTAAAGGAAGGGAAATCAACCGAGACCCCGT350.2638920304606801No Hit
GGCATTGAGAAGGAAGGACGCTTTCAGAGGCGA340.2563522581618035No Hit
ACCGTATCCAAGCTCCGTGGCTAGTCTGCGCTC340.2563522581618035No Hit
AGTCTGCGCTCTTTGGACTTTGAAAACTTAGCG330.24881248586292692No Hit
GGATGGATGCCCGGGCATTGAGAAGGAAGGACG330.24881248586292692No Hit
GTTTATAGTCGCGACTCTTGTCATAGTCAAGAA330.24881248586292692No Hit
ATCCATGGATCTCCGATCGGGAAACCGTATCCA320.24127271356405036No Hit
ACCGAGACCCCGTTAGTAGCGGCGAGCGAGAGC300.2261931689662972No Hit
GTCTGCGCTCTTTGGACTTTGAAAACTTAGCGA290.21865339666742065No Hit
GGGGAGATACCGTCTGTGATCCATGGATCTCCG290.21865339666742065No Hit
GATACCGTCTGTGATCCATGGATCTCCGATCGG290.21865339666742065No Hit
TAGTAGCGGCGAGCGAGAGCGGATTTGGGATTT270.2035738520696675No Hit
CCATGGATCTCCGATCGGGAAACCGTATCCAAG260.19603407977079093No Hit
CTCCGTGGCTAGTCTGCGCTCTTTGGACTTTGA260.19603407977079093No Hit
GTGAATGGGCAACGAACAAAAAAAAAAAAAAAA250.18849430747191434No Hit
GTGAATGGGCAACGAACCAAAAAAAAAAAAAAA240.18095453517303778No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA230.17341476287416122No Hit
CCGTATCCAAGCTCCGTGGCTAGTCTGCGCTCT230.17341476287416122No Hit
GGCTAAATACGGGCGAGAGACCGATAGCGAACA230.17341476287416122No Hit
AACCAAGACAAAAAAAAAAAAAAAAAAAAAAAA220.16587499057528463No Hit
GGCCATGGGGAGATACCGTCTGTGATCCATGGA220.16587499057528463No Hit
CCCCGTTAGTAGCGGCGAGCGAGAGCGGATTTG220.16587499057528463No Hit
TATCAACGCAGAGTGAATGGGCAACGAACCAAG220.16587499057528463No Hit
AACCAAGACGTACTCAAAAAAAAAAAAAAAAAA220.16587499057528463No Hit
GAAATCAACCGAGACCCCGTTAGTAGCGGCGAG210.15833521827640804No Hit
CATTGGATGGATGCCCGGGCATTGAGAAGGAAG210.15833521827640804No Hit
GTATAGAGTCTGACACCTGCCCGGTGCTGGAAA190.1432556736786549No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA190.1432556736786549No Hit
GCCCCAATCGGGCGGTAAATTCCGTCCAAGGCT190.1432556736786549No Hit
ACGCAGAGTGAATGGGCAACGAACCAAAAAAAA180.13571590137977835No Hit
ATGGATCTCCGATCGGGAAACCGTATCCAAGCT180.13571590137977835No Hit
GTGATCCATGGATCTCCGATCGGGAAACCGTAT170.12817612908090176No Hit
CCCTAATATGAACCTTGAGTCATCAAAGCCTTT170.12817612908090176No Hit
TATCAACGCAGAGTGAATGGGGAATGCATTGGA170.12817612908090176No Hit
GTTTAAGCATATCAAAAAAAAAAAAAAAAAAAA170.12817612908090176No Hit
GGAAGGACGCTTTCAGAGGCGAAAGGCCATGGG170.12817612908090176No Hit
CCCCAGGTCAGGCGGGATTACCCGCTGAGTTTA170.12817612908090176No Hit
GATCTCCGATCGGGAAACCGTATCCAAGCTCCG170.12817612908090176No Hit
GGAATGCATTGGATGGATGCCCGGGCATTGAGA170.12817612908090176No Hit
GAATGCATTGGATGGATGCCCGGGCATTGAGAA160.12063635678202518No Hit
CGCTTTCAGAGGCGAAAGGCCATGGGGAGATAC160.12063635678202518No Hit
CTTTGAAAACTTAGCGAACTGAAACATCTAAGT160.12063635678202518No Hit
GAGTTTAAGCATATCAAAAAAAAAAAAAAAAAA160.12063635678202518No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA150.1130965844831486No Hit
GAAGGAAGGACGCTTTCAGAGGCGAAAGGCCAT150.1130965844831486No Hit
CGAGAGAGCAGAGCGTACCGCCCTGCCATAGTC150.1130965844831486No Hit
ACGCAGAGTGAATGGGCAACGAACAAAAAAAAA150.1130965844831486No Hit
TATCAACGCAGAGTGAATGGGCAACGAACAAAA150.1130965844831486No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA150.1130965844831486No Hit
GTATCAACGCAGAGTGAATGGGCAACGAACCAA140.10555681218427204No Hit
AATCAACCGAGACCCCGTTAGTAGCGGCGAGCG140.10555681218427204No Hit
AACCGAGACCCCGTTAGTAGCGGCGAGCGAGAG140.10555681218427204No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT140.10555681218427204No Hit
GTCTTGGTTCGTTGAAAAAAAAAAAAAAAAAAA140.10555681218427204No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGAAG405.352449E-727.024
GAGAAGG454.8081347E-827.025
GGCATTG405.352449E-727.019
GGGCATT405.352449E-727.018
CATTGAG405.352449E-727.021
TTGAGAA405.352449E-727.023
CGGGCAT405.352449E-727.017
CCGGGCA405.352449E-727.016
GCATTGA405.352449E-727.020
AGAAGGA501.3350655E-724.30000126
GAAGGAA501.3350655E-724.30000127
GGATGCC451.4961188E-624.010
CCCGGGC451.4961188E-624.015
GATGCCC451.4961188E-624.011
ATGGATG451.4961188E-624.08
ATGCCCG451.4961188E-624.012
TGGATGC451.4961188E-624.09
GCCCGGG451.4961188E-624.014
ATTGAGA503.7396203E-621.622
GATGGAT558.539046E-619.6363647
Produced by FastQC (version 0.11.2)