FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate8_24.35200000060828.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate8_24.35200000060828.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73514
Sequences flagged as poor quality0
Sequence length33
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCACACTACACACGTACTCTGCGTTGATAC4700.6393340044073238No Hit
CCACACTACACACGTACTCTGCGTTGATACCAC3840.5223494844519412No Hit
ACTCCACACTACACACGTACTCTGCGTTGATAC2040.2774981636150937No Hit
GCTCCACACTACACACGTACTCTGCGTTGATAC1810.24621160595260766No Hit
CTTCCACACTACACACGTACTCTGCGTTGATAC1690.2298881845634845No Hit
GTCCACACTACACACGTACTCTGCGTTGATACC1570.21356476317436138No Hit
TCTCCACACTACACACGTACTCTGCGTTGATAC1480.201322197132519No Hit
CATCCACACTACACACGTACTCTGCGTTGATAC1380.18771934597491635No Hit
CTCCACACTACACACGTACTCTGCGTTGATACC1360.18499877574339582No Hit
CGTCCACACTACACACGTACTCTGCGTTGATAC1180.16051364365971107No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGC940.12786680088146476No Hit
ATTCCACACTACACACGTACTCTGCGTTGATAC920.12514623064994423No Hit
CGTGTGTAGTGTGAAAAAAAAAAAAAAAAAAAA920.12514623064994423No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA910.12378594553418396No Hit
ATCCACACTACACACGTACTCTGCGTTGATACC900.1224256604184237No Hit
GTTCCACACTACACACGTACTCTGCGTTGATAC870.11834480507114291No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC850.11562423483962238No Hit
CTACACACGTACTCAAAAAAAAAAAAAAAAAAA840.11426394972386211No Hit
GATCCACACTACACACGTACTCTGCGTTGATAC760.10338166879778No Hit
GGTCCACACTACACACGTACTCTGCGTTGATAC760.10338166879778No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGTA200.00856631926.99523719
ACACCCG200.00856631926.99523717
CAATATT257.7658956E-426.9952374
ATTGAAT200.00856631926.9952373
ATACCAC504.132109E-621.6108927
TTGATAC3550.019.40414427
GTATATA350.005468763419.2954391
TATCAAC1250.018.3692591
ATCAACG1201.8189894E-1217.9968262
GATACCA552.1041973E-417.19048326
GTTGATA4100.016.80115126
TCAACGC1351.6370905E-1115.997183
CAACGCA1402.910383E-1115.425854
GCCTCCA550.004195956514.7246757
CGTTGAT4800.013.78819925
CCTTCCA956.929478E-512.7872186
ATGGGAC750.00221996112.5977783
TCTGCGT15550.012.50791227
GCGTTGA5350.012.118257524
AACGCAG1801.6152626E-911.9978835
Produced by FastQC (version 0.11.2)