FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate8_22.352000000607e3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate8_22.352000000607e3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42791
Sequences flagged as poor quality0
Sequence length33
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAATGGGTAGTTCTGACACAAAAAAAAAAAA2540.5935827627304807No Hit
TATCAACGCAGAGTGAATGGGTAGTTCTGACAC2440.5702133626229814No Hit
GTTCTGACACGTACAAAAAAAAAAAAAAAAAAA2380.5561917225584819No Hit
GTTCTGACACGTACTCAAAAAAAAAAAAAAAAA1740.4066275618704868No Hit
GGGTAGTTCTGACACGTACTCTGCGTTGATACC1500.35054100161248863No Hit
CGGTAGTTCTGACACGTACTCTGCGTTGATACC1430.33418242153723915No Hit
ACGCAGAGTGAATGGGTAGTTCTGACACAAAAA1180.27575892126849105No Hit
GTATCAACGCAGAGTGAATGGGTAGTTCTGACA1110.25940034119324157No Hit
TATCAACGCAGAGTGAATGGGGTAGTTCTGACA1050.24537870112874205No Hit
GTGAATGGGGTAGTTCTGACACAAAAAAAAAAA810.18929214087074384No Hit
GTGAATGGGGTAGTTCAAAAAAAAAAAAAAAAA800.18695520085999393No Hit
TATCTACGCAGAGTGAATGGGTAGTTCTGACAC750.17527050080624432No Hit
GGGTAGTTCTGACACAAAAAAAAAAAAAAAAAA720.16825968077399453No Hit
GTATCATCGCAGAGTGAATGGGTAGTTCTGACA710.16592274076324462No Hit
GTTCTGACACAAAAAAAAAAAAAAAAAAAAAAA700.16358580075249468No Hit
AACGCAGAGTGAATGGGTAGTTCTGACACAAAA690.16124886074174474No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGC660.15423804070949498No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA630.14722722067724522No Hit
GTGAATGGGTAGTTCAAAAAAAAAAAAAAAAAA630.14722722067724522No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC600.14021640064499546No Hit
GAGTGAATGGGTAGTTCTGACACAAAAAAAAAA590.13787946063424553No Hit
GTGAATGGGTAGTTCTGACACGTACAAAAAAAA590.13787946063424553No Hit
GTTCTGACACGTAAAAAAAAAAAAAAAAAAAAA580.1355425206234956No Hit
AGGTAGTTCTGACACGTACTCTGCGTTGATACC580.1355425206234956No Hit
CGTACTCAAAAAAAAAAAAAAAAAAAAAAAAAA570.13320558061274565No Hit
CGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAA550.12853170059124583No Hit
CCCCCCGGTAGTTCTGACACGTACTCTGCGTTG530.12385782056974597No Hit
GTGAATGGGTAGTTCTGACACGAAAAAAAAAAA490.11451006052674627No Hit
GTGAATGGGTAGTTCTGACACGTACTCAAAAAA460.1074992404944965No Hit
AACGCAGAGTGAATGGGTAGTTCTGACACGTAC450.10516230048374657No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCAG405.852871E-727.05
CTACGCA405.852871E-727.04
TGGGAAG200.00851947827.018
ATCTACG451.634764E-624.0000022
TATCTAC451.634764E-624.0000021
AGTACGT300.002229361822.5000022
TCTACGC504.0830964E-621.63
TGCGTTG3700.018.24324227
GGTATCA500.002200303416.21
AATGGGA707.3969975E-515.4285724
GTATCAA1302.59206E-914.5384621
TATCAAC2200.014.1136371
ACCGGTA600.007395764313.5000016
GCGTTGA1202.3060966E-713.50000127
ATGGGGG750.002190716612.612
TCTGCGT11100.012.52702727
ATCAACG2300.012.3260872
ACGTACA1001.0965612E-412.1500019
CTGGTAG906.227909E-412.0000017
TCAACGC2400.011.8125013
Produced by FastQC (version 0.11.2)