FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate8_21.352000000607c9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate8_21.352000000607c9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37628
Sequences flagged as poor quality0
Sequence length33
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA940.24981396832146277No Hit
GGTACGTACTCTGCGTTGATACAAAAAAAAAAA820.21792282342936112No Hit
GTTCAAGGTAAAAAAAAAAAAAAAAAAAAAAAA750.19931965557563516No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA700.1860316785372595No Hit
GTTCAAGGTACGTACTCAAAAAAAAAAAAAAAA700.1860316785372595No Hit
GTACGTACTCTGCGTTGATACAAAAAAAAAAAA630.16742851068353354No Hit
TATCAACGCAGAGTGAATGGGTACGTACTCTGC620.1647709152758584No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT590.156798129052833No Hit
GTGAATGGGTACGTACTCTGCGTTGATACAAAA580.15414053364515787No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA570.1514829382374827No Hit
ACTCTGCGTTGATACAAAAAAAAAAAAAAAAAA520.13819496119910704No Hit
CCCCAGTTCAAGGTACGTACTCTGCGTTGATAC490.13022217497608166No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA480.1275645795684065No Hit
GCGTTGATACAAAAAAAAAAAAAAAAAAAAAAA440.11693419793770597No Hit
GTTCAAGGAAAAAAAAAAAAAAAAAAAAAAAAA440.11693419793770597No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTACG200.00849825327.0053312
GTATCAT257.6778285E-427.0053311
TCTACGC200.00849825327.0053313
TACGCAG200.00849825327.0053315
TATCATC257.6778285E-427.0053312
ATCATCG257.6778285E-427.0053313
CTACGCA200.00849825327.0053314
TCTCCCA200.00849825327.0053315
TCATCGC257.6778285E-427.0053314
GATCCAG200.008553809526.9693746
CGGCCAG200.008553809526.9693746
TCGCAGA257.738031E-426.9693727
ATCGCAG257.738031E-426.9693726
TAGCCAG352.0037763E-423.1166066
CATCGCA300.002221767622.5044445
TCTGCGT16250.020.4409627
CCCTCCA404.897408E-420.2545
CACACCA350.005429512419.2895245
AAACCAG350.00547162619.2638386
ACGCCAG350.00547162619.2638386
Produced by FastQC (version 0.11.2)